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Team:Chiba/AiiA Receiver Phase
From 2008.igem.org
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==AiiA Receiver== | ==AiiA Receiver== | ||
| - | [[Image:AiiA-Receiver Chiba.gif|frame|right|'''Fig. | + | [[Image:AiiA-Receiver Chiba.gif|frame|right|'''Fig.''' AiiA Receiver]] |
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===Design=== | ===Design=== | ||
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<br clear=all> | <br clear=all> | ||
| - | + | [[Image:Aiia reaction chiba.gif|frame|left|'''Fig.''' AiiA Reaction]] | |
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===Experiment=== | ===Experiment=== | ||
*'''Sender''' | *'''Sender''' | ||
Revision as of 11:17, 29 October 2008
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AiiA Receiver
Fig. AiiA Receiver
Design
オートインデューサー不活性化酵素であるAiiAをレシーバー菌で発現させることにより、レシーバー菌内でのAHL濃度の増加が遅くなるため、AiiAを発現しないレシーバー菌に比べて、GFP発現が遅くなる。（小林）
Fig. AiiA Reaction
Experiment
- Sender
- [http://partsregistry.org/Part:BBa_S03623 BBa_S03623 (AHL autoinucer)]
- Receivers
- AiiA Receiver
- non-AiiA Receiver
result
Fig.Time Delay Test
24時間後の蛍光強度を比較すると最大強度は1/4に下がっている。
transfer curveがシグモイドではなく、時間に比例するようになった
AiiAを発現させると、GFPの発現自体が極端に減ってしまったので、AHL自体を減らしてしまうと発現量の最大値が小さくなってしまう。
conclusion
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