Wiki/Team:Warsaw/igem notebook.htm

From 2008.igem.org

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Revision as of 13:38, 29 October 2008

Gallery Bricks Notebook Team Project Home

Table of contents

 

Preparation of one-armed bandit vectors

One-armed bandit tests

Preparation of vectors for 'hunter' part of 'hunter and prey'

Preparation of vectors for 'prey' part of 'hunter and prey'

Purification of prey proteins

'Hunter and prey' tests

Preparation of BioBricks

Other activities

May
MTWTFSS
      [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_May_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_May_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_May_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_May_2008 4]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/5_May_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_May_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_May_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_May_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_May_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_May_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_May_2008 11]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/12_May_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_May_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_May_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_May_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_May_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_May_2008 18]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/19_May_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_May_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_May_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_May_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_May_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_May_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_May_2008 25]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/26_May_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_May_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_May_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_May_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_May_2008 30] [http://2008.igem.org/Team:Warsaw/Calendar-Main/31_May_2008 31]
June
MTWTFSS
            [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_June_2008 1]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/2_June_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_June_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_June_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_June_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_June_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_June_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_June_2008 8]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/9_June_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_June_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_June_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_June_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_June_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_June_2008 15]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/16_June_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_June_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_June_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_June_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_June_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_June_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_June_2008 22]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/23_June_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_June_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_June_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_June_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_June_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_June_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_June_2008 29]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/30_June_2008 30]
July
MTWTFSS
  [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_July_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_July_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_July_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_July_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_July_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_July_2008 6]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/7_July_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_July_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_July_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_July_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_July_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_July_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_July_2008 13]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/14_July_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_July_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_July_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_July_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_July_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_July_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_July_2008 20]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/21_July_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_July_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_July_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_July_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_July_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_July_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_July_2008 27]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/28_July_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_July_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_July_2008 30] [http://2008.igem.org/Team:Warsaw/Calendar-Main/31_July_2008 31]
August
MTWTFSS
        [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_August_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_August_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_August_2008 3]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/4_August_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_August_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_August_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_August_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_August_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_August_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_August_2008 10]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/11_August_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_August_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_August_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_August_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_August_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_August_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_August_2008 17]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/18_August_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_August_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_August_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_August_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_August_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_August_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_August_2008 24]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/25_August_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_August_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_August_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_August_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_August_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_August_2008 30] [http://2008.igem.org/Team:Warsaw/Calendar-Main/31_August_2008 31]
September
MTWTFSS
[http://2008.igem.org/Team:Warsaw/Calendar-Main/1_September_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_September_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_September_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_September_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_September_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_September_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_September_2008 7]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/8_September_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_September_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_September_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_September_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_September_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_September_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_September_2008 14]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/15_September_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_September_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_September_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_September_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_September_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_September_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_September_2008 21]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/22_September_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_September_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_September_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_September_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_September_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_September_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_September_2008 28]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/29_September_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_September_2008 30]
October
MTWTFSS
    [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_October_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_October_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_October_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_October_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_October_2008 5]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/6_October_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_October_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_October_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_October_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_October_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_October_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_October_2008 12]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/13_October_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_October_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_October_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_October_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_October_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_October_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_October_2008 19]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/20_October_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_October_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_October_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_October_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_October_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_October_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_October_2008 26]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/27_October_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_October_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_October_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_October_2008 30] [http://2008.igem.org/Team:Warsaw/Calendar-Main/31_October_2008 31]
November
MTWTFSS
          [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/1_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/2_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 2]
[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/3_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/4_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/5_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/6_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_November_2008 7] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/8_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/9_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 9]
[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/10_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/11_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/13_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/14_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/15_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/16_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 16]
[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/17_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/18_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_November_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_November_2008 20] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/21_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/22_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/23_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 23]
[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/24_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/25_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/26_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_November_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_November_2008 28] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/29_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/30_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 30]

Protocols

Primers

Vectors

JavaScript tools developed to make working with iGEM notebook more comfortable

Strains

Rosetta(DE3)pLysS

F- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)

TOP10 (Invitrogen)

F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ-

NR9458 (IBB collection; in notebook called mutD5)

ara thi Δ pro lac mutD5 zaf-13 ::Tn10

GM2163

ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2
Retrieved from "http://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm"