Lux signaling system

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Revision as of 14:40, 29 October 2008

Lux cell signaling system

Responsible: Andrew Gordon and Pallavi Penumetcha

Signal molecule: N-acyl-homoserine lactone (AHL) Generic term for a variety of species specific hormone-like molecules

Bacterial species: discovered in Vibrio fischeri known to work in E. coli

Receiver protein: LuxR protein receives signal from AHL; also has some control over transciption of luciferase

Signal molecule synthase: LuxI; also has some control over transciption of luciferase

Additional Information: "Quorum Quenching" aiiA (intracellular) lactonase reduces AHL concentration

800px-Luxrreceiverschematic.png

Resources

[http://partsregistry.org/Lux Lux Operon Pathway]

[http://partsregistry.org/AHL AHL signaling molecules by species; some are specific to gram pos but may affect gram negs]

[http://www.horizonpress.com/jmmb/v1/v1n1/03.pdf Reducing Crosstalk in Lux System]

References

[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=522112 Quorum Quenching to control Lux Pathway]

[http://www.nature.com/nature/journal/v434/n7037/full/nature03461.html] A Synthetic multicellular system for programmed pattern formation

Parts Needed:

LuxR + pro/term

RBS

LuxI + pro/term

LuxI sender

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