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Team:Chiba/Sender experiments/Senders(XL10Gold) T9002(JW1908)
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[[Image:Chiba_talks_XL10Gold_25_RS1_01.gif|thumb|left|Fig.<br>E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,25°C,Receiver cells/Sender cells = 1.]] | [[Image:Chiba_talks_XL10Gold_25_RS1_01.gif|thumb|left|Fig.<br>E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,25°C,Receiver cells/Sender cells = 1.]] | ||
[[Image:Chiba_talks_XL10Gold_25_RS1_02.gif|thumb|left|Fig.<br> E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,25°C,Receiver cells/Sender cells = 1.]] | [[Image:Chiba_talks_XL10Gold_25_RS1_02.gif|thumb|left|Fig.<br> E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,25°C,Receiver cells/Sender cells = 1.]] | ||
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#蛍光強度が、30°C,37°Cに比べて極端に低く,Sendersの培養液を添加しなかった反応液と差がほとんどなかった.LuxI,LasI,RhlI,LuxR,GFPすべての発現量が減っていたためと考えられる. | #蛍光強度が、30°C,37°Cに比べて極端に低く,Sendersの培養液を添加しなかった反応液と差がほとんどなかった.LuxI,LasI,RhlI,LuxR,GFPすべての発現量が減っていたためと考えられる. | ||
| - | + | #LuxI,RhlIの培養液を,混ぜた反応液に比べて,LasIの培養液を混ぜた反応液は, | |
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[[Team:Chiba/Sender experiments#Experiment|Back to Sender experiment and result]] | [[Team:Chiba/Sender experiments#Experiment|Back to Sender experiment and result]] | ||
Revision as of 14:49, 29 October 2008
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design
Sender
- [http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI(BBa_K084007)]
- [http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI(BBa_K084008)]
- [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
- [http://partsregistry.org/Part:BBa_K084012 BBa_K084012]
- [http://partsregistry.org/Part:BBa_K084014 BBa_K084014]
- [http://partsregistry.org/Part:BBa_S03623 BBa_S03623(ptet+rbs+LuxI(LVA))]
Receiver
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]
Method
- Transformed Senders into E.coli strains(JW1908) and Receiver into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 37°C.
- Measured intensity of green fluorescence at regular time intervals.
Results
Reaction temparature:37°C
- センダーの培養液：500μL、レシーバの培養液：500μL
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Reaction temparature:30°C
- センダーの培養液：500μL、レシーバの培養液：500μL
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Reaction temparature:25°C
センダーの培養液：500μL、レシーバの培養液：500μL
E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,25°C,Receiver cells/Sender cells = 1.
E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,25°C,Receiver cells/Sender cells = 1.
Left:
- 蛍光強度が、30°C,37°Cに比べて極端に低く,Sendersの培養液を添加しなかった反応液と差がほとんどなかった.LuxI,LasI,RhlI,LuxR,GFPすべての発現量が減っていたためと考えられる.
- LuxI,RhlIの培養液を,混ぜた反応液に比べて,LasIの培養液を混ぜた反応液は,
Back to Sender experiment and result
