IGEM:Cambridge/2008/Notebook/Voltage/Output

From 2008.igem.org

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*Lower probe electrode into cell solution and wait for stable reading - 1min.
*Lower probe electrode into cell solution and wait for stable reading - 1min.
*Add sample solution
*Add sample solution
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[[Image:Voltage_apparatus.jpg|800px| Apparatus setup]]
+
[[Image:Voltage_apparatus.jpg|500px| Apparatus setup]]
=Results=
=Results=

Revision as of 17:02, 29 October 2008

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Setup

Apparatus is setup as shown. An earth is needed next to the probe electrode, but not touching. They will be needed within the same solution to reduce the noise.

  • Cells containing biobrick K090002 are grown overnight in NOK with 5%(by vol.) 2M KCl added - final 100mM K+.
  • Samples are then spun down in individual 1.5ml eppendorfs.

Experiment

  • Resuspend cells in 200μL clean NOK and place in sample wells
  • Lower probe electrode into cell solution and wait for stable reading - 1min.
  • Add sample solution

Apparatus setup

Results

Output at different GluR0 concentrations

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