Team:Chiba/Sender experiments/Senders(XL10Gold) T9002(JW1908)

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Revision as of 17:44, 29 October 2008

Chiba-U.gif

Home The Team The Project Parts Submitted to the Registry Reference Notebook Acknowledgements


design

Sender

  • [http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI(BBa_K084007)]

Tet-lasi chiba.gif

  • [http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI(BBa_K084008)]

Tet-rhli chiba.gif

  • [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]

Tet-rhli chiba.gif

  • [http://partsregistry.org/Part:BBa_K084012 BBa_K084012]

Tet-luxi chiba.gif

  • [http://partsregistry.org/Part:BBa_K084014 BBa_K084014]

Tet-luxi chiba.gif

  • [http://partsregistry.org/Part:BBa_S03623 BBa_S03623(ptet+rbs+LuxI(LVA))]

Chiba BBa S03623.gif

Receiver

  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]

Reporter 9002 chiba.gif

Method

  1. Transformed Senders into E.coli strains(JW1908) and Receiver into E.coli strain(JW1908).
  2. Inoculated them independently in liquid media. Incubated at 37°C 12h
  3. Mixed them.
  4. Incubated at 37°C.
  5. Measured intensity of green fluorescence at regular time intervals.

more details...

Results

Reaction temparature:37°C

  • センダーの培養液：500μL、レシーバの培養液：500μL
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.


Reaction temparature:30°C

  • センダーの培養液：500μL、レシーバの培養液：500μL
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.


Reaction temparature:25°C

センダーの培養液：500μL、レシーバの培養液：500μL

Fig.
E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,25°C,Receiver cells/Sender cells = 1.
Fig.
　E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,25°C,Receiver cells/Sender cells = 1.


Left:

  1. 蛍光強度が、30&degC,37&degCに比べて極端に低く,Sendersの培養液を添加しなかった反応液と差がほとんどなかった.LuxI,LasI,RhlI,LuxR,GFPすべての発現量が減っていたためと考えた.
  2. LuxI,RhlIの培養液を,混ぜた反応液に比べて,LasIの培養液を混ぜた反応液は,最大蛍光強度が小さかった(約1/2).

Right:

  1. RhlIにLVAtagのついているものは,LVAtagのついていないものに比べて最大蛍光強度が小さかった.



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