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Template:Team:UC Berkeley/Notebook/SC notes
From 2008.igem.org
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(We also learned how to use the "spreader method" without setting the ethanol on fire....) | (We also learned how to use the "spreader method" without setting the ethanol on fire....) | ||
| - | We | + | We finished at 8:15p.m. |
Revision as of 18:03, 26 June 2008
23 June 2008 (M)
Having completed one week of training/lectures, I've finally started on my minute portion of the lab work! Over last weekend, I searched for the DNA sequences of the FLAG-tag and the AP-tag. The objective was to find an existing E. coli vector for said sequences. Upon completing the search, I designed oligos for EIPCR for small parts under 30 bp (like our tags). When we discovered, however, that our sequences were too long - leaning precariously over the "30bp border" - we decided to prepare for a Wobble (overlap extension) PCR instead. This involved designing Fwd/Rev oligos that had a 20bp overlap. When that was done, we ordered the oligos. We now eagerly expect their arrival tomorrow at around 3p.m., when we can actually do the PCR reation!
24 June 2008 (T)
I finished creating/organizing my oligos and construction files onto spreadsheets and documents.
We did set up for the wobble PCRs; they're in the thermocycler overnight until tomorrow!
25 June 2008 (W)
Rundown of the busy schedule we had today:
1. Purified the PCR product (using Zymo cleanup)
2. Set up for restriction digest
3. Clean up (Zymo) the digest
4. Set up for Digestion
5. Transformation (We also learned how to use the "spreader method" without setting the ethanol on fire....)
We finished at 8:15p.m.
