Team:Chiba/Calendar-Home/14 September 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Output)
Line 41: Line 41:
*[http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
*[http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
*[http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
*[http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
-
*[http://partsregistry.org/Part:BBa_I7100 BBa_I7100](x2)
+
*[http://partsregistry.org/Part:BBa_I7100 BBa_I7100](×2)
*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
Line 50: Line 50:
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(digested sample)(μL)</td>
+
<td>DNA(digested sample)(&mu;L)</td>
<td>3</td>
<td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer2(x10)(μL)</td>
+
<td>Buffer2(x10)(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O(μL)</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>6</td>
<td>6</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ(μL)</td>
+
<td>EcoRⅠ(&mu;L)</td>
<td>0.075</td>
<td>0.075</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL(μL)</td>
+
<td>TOTAL(&mu;L)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
</table>
</table>
-
--> left at37 degrees for 1 hour  
+
--> left at 37&deg;C for 1 hour  
[[Team:Chiba/protocol/gelcheck|Gel check]]
[[Team:Chiba/protocol/gelcheck|Gel check]]
Line 81: Line 81:
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(digestion sample)(μL)</td>
+
<td>DNA(digestion sample)(&mu;L)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye(μL)</td>
+
<td>Loading Dye(&mu;L)</td>
<td>2</td>
<td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL(μL)</td>
+
<td>TOTAL(&mu;L)</td>
<td>12</td>
<td>12</td>
</tr>
</tr>
</table>
</table>

Revision as of 19:43, 29 October 2008

>Home | Notebook

13 September 2008 <|> 15 September 2008

Laboratory work

Team:Communication

(13/9)--->Mini prep


Transformation
Competent cells : XL10Gold
  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084009 BBa_K084009](Plac+RhlI+LVA)
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA)
--->(15/9)


Team:Output

Time Responce Liquid

Digestion test

  • [http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]
  • [http://partsregistry.org/Part:BBa_I716463 BBa_I716463]
  • [http://partsregistry.org/Part:BBa_C0051 BBa_C0051]
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070]
  • [http://partsregistry.org/Part:BBa_J06620 BBa_J06620]
  • [http://partsregistry.org/Part:BBa_J22141 BBa_J22141]
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
  • BBa_I716542
  • [http://partsregistry.org/Part:BBa_I716462 BBa_I716462]
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078]
  • [http://partsregistry.org/Part:BBa_J01101 BBa_J01101]
  • [http://partsregistry.org/Part:BBa_C0071 BBa_C0071]
  • [http://partsregistry.org/Part:BBa_I712019 BBa_I712019]
  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
  • [http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
  • [http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
  • [http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
  • [http://partsregistry.org/Part:BBa_I7100 BBa_I7100](×2)
  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
Sample No. each
DNA(digested sample)(μL) 3
Buffer2(x10)(μL) 1
dH2O(μL) 6
EcoRⅠ(μL) 0.075
TOTAL(μL) 10

--> left at 37°C for 1 hour

Gel check

Sample No. each
DNA(digestion sample)(μL) 10
Loading Dye(μL) 2
TOTAL(μL) 12
Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/14_September_2008"