Team:Chiba/Calendar-Home/18 September 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Communication)
Line 12: Line 12:
***[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
***[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
**E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
**E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
-
**temparature<br>37°C and 30°C
+
**temparature<br>37&deg;C and 30&deg;C
===Team:Output===
===Team:Output===
Line 19: Line 19:
->left for:
->left for:
-
#1 hour at 37 degrees
+
#1 hour at 37 &deg;C
-
#30 minutes at 65 degrees.
+
#30 minutes at 65 &deg;C
[[Team:Chiba/protocol/transformation|Zymoclean]]  
[[Team:Chiba/protocol/transformation|Zymoclean]]  
-
->eluted with 25μL of NFW.  
+
->eluted with 25&mu;L of NFW.  
[[Team:Chiba/protocol/ligation/ligation|Ligation]]
[[Team:Chiba/protocol/ligation/ligation|Ligation]]
Line 37: Line 37:
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(vector)(μL)</td>
+
<td>DNA(vector)(&mu;L)</td>
<td>3.5</td><td>3.5</td>
<td>3.5</td><td>3.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(insert)(μL)</td>
+
<td>DNA(insert)(&mu;L)</td>
<td>7.5</td><td>-</td>
<td>7.5</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Ligase(μL)</td>
+
<td>Ligase(&mu;L)</td>
<td>1</td><td>1</td>
<td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Ligase Buffer(μL)</td>
+
<td>Ligase Buffer(&mu;L)</td>
<td>4</td><td>4</td>
<td>4</td><td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O(μL)</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>4</td><td>4</td>
<td>4</td><td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL(μL)</td>
+
<td>TOTAL(&mu;L)</td>
<td>20</td><td>20</td>
<td>20</td><td>20</td>
</tr>
</tr>
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</tr>
</tr>
<tr>
<tr>
-
<td>DNA(μL)</td>
+
<td>DNA(&mu;L)</td>
<td>each</td>
<td>each</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye(μL)</td>
+
<td>Loading Dye(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O(μL)</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>4</td>
<td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL(μL)</td>
+
<td>TOTAL(&mu;L)</td>
<td>6</td>
<td>6</td>
</tr>
</tr>
</table>
</table>

Revision as of 19:50, 29 October 2008

>Home | Notebook

17 September 2008 <|> 19 September 2008

Laboratory work

Team:Communication

  • time-delay test
    • transformants
      • [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
      • [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
      • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
    • E.coli strain
      Senders:XL10GOLD
      Receiver:JW1908
    • temparature
      37°C and 30°C

Team:Output

SAP 1μL

->left for:

  1. 1 hour at 37 °C
  2. 30 minutes at 65 °C

Zymoclean

->eluted with 25μL of NFW.

Ligation

  • vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
  • insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
  • Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR)
Sample No. Vector&insertVector
DNA(vector)(μL) 3.53.5
DNA(insert)(μL) 7.5-
Ligase(μL) 11
Ligase Buffer(μL) 44
dH2O(μL) 44
TOTAL(μL) 2020

transformation

  • [http://partsregistry.org/Part:BBa_J54103 BBa_J54103](RBS+GFP)
  • [http://partsregistry.org/Part:BBa_P2006 BBa_P2006]

Gel check

  • Ligation product(Venus YFP)
  • [http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
  • [http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
  • [http://partsregistry.org/Part:BBa_J37032 BBa_J37032]
Sample No. each
DNA(μL) each
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
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