Team:Chiba/Calendar-Home/24 August 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Output)
(→Team:Communication)
Line 42: Line 42:
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ</td>
+
<td>EcoRI</td>
<td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
<td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
</tr>
</tr>
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<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>10</td><td>10</td><td>10</td><td>10</td>
+
<td>10μl</td><td>10μl</td><td>10μl</td><td>10μl</td>
</tr>
</tr>
</table>
</table>
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<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>6</td>
+
<td>6μl</td>
</tr>
</tr>
</table>
</table>
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<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>12</td>
+
<td>12μl</td>
</tr>
</tr>
</table>
</table>

Revision as of 20:00, 29 October 2008

>Home | Notebook

23 August 2008 <|> 25 August 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(22/8)--->Mini prep
  1. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)
  3. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)
  4. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006)


--->Digestion test
  1. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)
  3. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)
  4. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006)


Sample No. 5678
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) 1---
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -1--
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) --1-
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) ---1
EcoRI 0.10.10.10.1
Buffer 4 0.90.90.90.9
dH2O 8888
TOTAL 10μl10μl10μl10μl


--->Gel Check
Chiba-0824-1.JPG
Sample No. 1~8
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μl
From left;
  1. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> ?
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -> OK
  3. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) -> OK
  4. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006) -> OK
  5. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
  6. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -> OK
  7. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) -> OK
  8. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006) -> None?
Chiba-0824-2.JPG
Sample No. 5~8
Sample DNA 9
Loading Dye 2
dH2O 1
TOTAL 12μl

From the left; （single digestuion）

5, [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
6, [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -> OK
7, [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) -> 3400bp:Bad, 2200bp:OK
8, [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006) -> OK

Team:Output

Transformation

  • [http://partsregistry.org/Part:BBa_J63001 BBa_J63001]-->colony

Digestion

  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](1)①
  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2)②
  • Menbren Venus YFP③
Sample No. ①②③
DNA template 333
Buffer3 111
dH2O 666
EcoRⅠ 0.50.50.5
TOTAL 10μl10μl10μl


-->37°C 30min


Sample No. ①(afetr digestion)②(after digestion)③(afetr digestion)
DNA tamplate 101010
loading Dye 222
TOTAL 12μl12μl12μl


Sample No. ①②③
DNA tamplate 222
loading Dye 111
dH2O 333
TOTAL 6μl6μl6μl

-->135V 30min

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