Team:Chiba/Calendar-Home/24 August 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Communication)
(→Team:Output)
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[[Team:Chiba/protocol/digestion|Digestion]]
[[Team:Chiba/protocol/digestion|Digestion]]
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*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](1)①
+
*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](1)
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*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2)②
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*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2)
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*Menbren Venus YFP③
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*Menbren Venus YFP(3)
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
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<td>①</td><td>②</td><td>③</td>
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<td>1</td><td>2</td><td>3</td>
</tr>
</tr>
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<tr>
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</tr>
</tr>
<tr>
<tr>
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<td>EcoRⅠ</td>
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<td>EcoRI</td>
<td>0.5</td><td>0.5</td><td>0.5</td>
<td>0.5</td><td>0.5</td><td>0.5</td>
</tr>
</tr>
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<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
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<td>①(afetr digestion)</td><td>②(after digestion)</td><td>③(afetr digestion)</td>
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<td>1(afetr digestion)</td><td>2(after digestion)</td><td>3(afetr digestion)</td>
</tr>
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<tr>
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<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
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<td>①</td><td>②</td><td>③</td>
+
<td>1</td><td>2</td><td>3</td>
</tr>
</tr>
<tr>
<tr>

Revision as of 20:02, 29 October 2008

>Home | Notebook

23 August 2008 <|> 25 August 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(22/8)--->Mini prep
  1. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)
  3. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)
  4. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006)


--->Digestion test
  1. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)
  3. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)
  4. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006)


Sample No. 5678
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) 1---
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -1--
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) --1-
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) ---1
EcoRI 0.10.10.10.1
Buffer 4 0.90.90.90.9
dH2O 8888
TOTAL 10μl10μl10μl10μl


--->Gel Check
Chiba-0824-1.JPG
Sample No. 1~8
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μl
From left;
  1. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> ?
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -> OK
  3. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) -> OK
  4. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006) -> OK
  5. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
  6. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -> OK
  7. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) -> OK
  8. [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006) -> None?
Chiba-0824-2.JPG
Sample No. 5~8
Sample DNA 9
Loading Dye 2
dH2O 1
TOTAL 12μl

From the left; （single digestuion）

5, [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
6, [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -> OK
7, [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) -> 3400bp:Bad, 2200bp:OK
8, [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006) -> OK

Team:Output

Transformation

  • [http://partsregistry.org/Part:BBa_J63001 BBa_J63001]-->colony

Digestion

  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](1)
  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2)
  • Menbren Venus YFP(3)
Sample No. 123
DNA template 333
Buffer3 111
dH2O 666
EcoRI 0.50.50.5
TOTAL 10μl10μl10μl


-->37°C 30min


Sample No. 1(afetr digestion)2(after digestion)3(afetr digestion)
DNA tamplate 101010
loading Dye 222
TOTAL 12μl12μl12μl


Sample No. 123
DNA tamplate 222
loading Dye 111
dH2O 333
TOTAL 6μl6μl6μl

-->135V 30min

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