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User:University of Washington/26 June 2008
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== Yeast Shuttle Plasmid == | == Yeast Shuttle Plasmid == | ||
| - | - | + | - Yeast growth media YEPD was obtained. |
| - | - | + | - Yeast transforming media 50% PEG, 10x LiAc, and 10x TE were obtained. |
| - | - | + | - Isolated yeast colonies were inoculated in 5 mL of YEPD media and incubated overnight at 30 degrees Celsius in a rotator. |
== Conjugation Plasmid == | == Conjugation Plasmid == | ||
Revision as of 22:48, 26 June 2008
Contents |
Yeast Shuttle Plasmid
- Yeast growth media YEPD was obtained.
- Yeast transforming media 50% PEG, 10x LiAc, and 10x TE were obtained.
- Isolated yeast colonies were inoculated in 5 mL of YEPD media and incubated overnight at 30 degrees Celsius in a rotator.
Conjugation Plasmid
Mini Preps
·C600+RP4 construct
Glycerol Stocks
·C600+RP4
·S17-1
LuxR from AraC and TetR
- Got 1 colony for TetR, 24 for AraC
- Grew culture for Glycerol Stock and Miniprep
LuxR from pLac
- Got 13 colonies for R0010, and 20 colonies for J04430. I0462 failed to grow.
- Grew cultures of R0010 and J04430 for glycerol stock. I0462 transformed e.coli sat out overnight in non-selective media but were plated again on Amp anyway to see if anything would grow.
- Four colonies from the J04430 plate were selected, and re-plated on Amp agar for later use.
Team:University_of_Washington/Notebook#Notebook
