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Team:Imperial College/Biobricks
From 2008.igem.org
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{{Imperial/Box2|Biobrick Characterisation in ''B.subtilis''| | {{Imperial/Box2|Biobrick Characterisation in ''B.subtilis''| | ||
This year Imperial College has added 45 new ''B.subtilis'' specific parts to the registry. In addition we sort to characterise some of these parts, including the constitutive promoter and ribosome binding site '''pveg-spoVG'''. The testing constructs we developed for this are shown below: | This year Imperial College has added 45 new ''B.subtilis'' specific parts to the registry. In addition we sort to characterise some of these parts, including the constitutive promoter and ribosome binding site '''pveg-spoVG'''. The testing constructs we developed for this are shown below: | ||
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| + | {{Imperial/Box1|Summary of Data| | ||
| + | The promoter and RBS combinations were characterised by measuring the steadty-state levels expression of fluorescent proteins in ''B.subtilis''. Cultures of ''B.subtilis'' transformed with the test constructs and non-transformed ''B.subtilis'' (control) were grown to the mid-log phase and measured using a plate reader. | ||
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| + | [[Image:Biobricks Imperial.PNG|center|500px]] | ||
[[Image:Fluorescence B.subtilis1.PNG]] | [[Image:Fluorescence B.subtilis1.PNG]] | ||
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Revision as of 20:37, 29 October 2008
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