Team:Imperial College/Biobricks
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{{Imperial/Box1|Summary of Data| | {{Imperial/Box1|Summary of Data| | ||
- | The promoter and RBS combinations were characterised by measuring the steadty-state levels expression of fluorescent proteins in ''B.subtilis''. Cultures of ''B.subtilis'' transformed with the test constructs and non-transformed ''B.subtilis'' (control) were grown to the mid-log phase and measured using a plate reader. | + | The promoter and RBS combinations were characterised by measuring the steadty-state levels expression of fluorescent proteins in ''B.subtilis''. Cultures of ''B.subtilis'' transformed with the test constructs and non-transformed ''B.subtilis'' (control) were grown to the mid-log phase. Fluorescence and O.D.<sub>600</sub> were both measured using a plate reader to generate fluorescence levels of the various samples. This fluorescence data was normalised based on cell density by using the O.D.<sub>600</sub> and a '''calibration curve of O.D.<sub>600</sub> vs colony forming units''' |
Revision as of 21:37, 29 October 2008
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