Team:Michigan/Project/LandingPads
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- | + | <font color=royalblue>Landing Pads:</font> aid in the insertion of biological constructs onto the chromosome of <i>E. coli</i> | |
+ | |||
+ | <font color=royalblue>Landing Pad Goals:</font> | ||
+ | * BioBrick compatibility | ||
+ | * Easy phenotypic screening | ||
+ | * Easy sequencing | ||
+ | * Limit noise | ||
+ | |||
+ | <font color=royalblue>Polylinker Features:</font> | ||
+ | * <font color=orangered>BioBrick compatible restriction sites</font> - these allow for standard BioBrick construction in forward and reverse directions | ||
+ | * <font color=lightseagreen>Additional building restriction sites</font> - these are for building the landing pad, as well as changing the homologous regions and drug resistance gene (can also be used for building if needed) | ||
+ | * <font color=firebrick>Bar code sequence</font> - a 20 bp segment that allows for easy sequencing | ||
+ | * <font color=blueviolet>Drug resistance gene</font> - this allows for easy phenotypic screening | ||
+ | * <font color=deeppink>Homologous regions</font> - these specify where on the chromosome you wish to put your constructs. | ||
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Revision as of 22:18, 29 October 2008
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BioBrick Landing PadsBioBrick Landing Pad Polylinker
Landing Pads used for the Sequestilator
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