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Minnesota/20 June 2008
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(New page: {| |- |1. '''Transformations:''' Transformations of genes/plasmids into cells and onto LB plates have grown. |- |2. '''Primers:''' Transferring from glass tubes to 1.5mL tubes. |NOTE:...)
Newer edit →
(New page: {| |- |1. '''Transformations:''' Transformations of genes/plasmids into cells and onto LB plates have grown. |- |2. '''Primers:''' Transferring from glass tubes to 1.5mL tubes. |NOTE:...)
Newer edit →
Revision as of 15:17, 27 June 2008
| 1. Transformations: Transformations of genes/plasmids into cells and onto LB plates have grown. | |
| 2. Primers: Transferring from glass tubes to 1.5mL tubes. | NOTE: Tube #12 looks darker than other primers possibly contaminated. |
| 3. Transformations continued: | |
| a. Thaw cells on ice | |
| b. Combine & incubate on ice (20 mins); 50uL of cells and
2uL plasmid. | |
| c. Heat shock 1 minute @ 42C. | |
| d. Incubate on ice for 5 minutes. | |
| e. Recover in 250uL S.O.C. @ 37C for 2 hours | |
| f. Plate 100uL and incubate O/N (overnight) @ 37C | |
| *Refer to iGEM “transformation” section for details. | NOTE: only #6, #12, and #13 plasmids/genes grew on Dh5alpha competent cells. The other eleven plasmids used had to be re-done for a transformation onto TOP10 competent cells. |
