Minnesota/23 June 2008

From 2008.igem.org

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|1. '''Continued plasmid prep work'''
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|2. '''Look up available Biobrick Plasmids'''
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|3. '''Begin iGEM wiki'''
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|4. '''Perform double digest.''' Double digest is when the plasmid is cut at two different restriction sites by using restriction enzymes.
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!| ||Double Digest ||4x ||10x
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| ||1.0 micrograms DNA ||2 ||1
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| ||(10x) Buffer 2 ||20 ||50
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| ||(100x) BSA  ||2 ||5
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| ||(20 units/uL) XBA1 ||2 ||5
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| ||(10 units/uL) Spe1 ||4 ||10
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| ||H20        ||164 ||420
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| ||Total sln      ||50 uL ||50 uL
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[[Image:6.23gel.jpg|700px||center|thumb|A gel run on 6/23]]
[[Image:6.23gel.jpg|700px||center|thumb|A gel run on 6/23]]

Revision as of 15:32, 27 June 2008

1. Continued plasmid prep work
2. Look up available Biobrick Plasmids
3. Begin iGEM wiki
4. Perform double digest. Double digest is when the plasmid is cut at two different restriction sites by using restriction enzymes.
Double Digest 4x 10x
1.0 micrograms DNA 2 1
(10x) Buffer 2 20 50
(100x) BSA 2 5
(20 units/uL) XBA1 2 5
(10 units/uL) Spe1 4 10
H20 164 420
Total sln 50 uL 50 uL







A gel run on 6/23
Retrieved from "http://2008.igem.org/Minnesota/23_June_2008"