Minnesota/24 June 2008

From 2008.igem.org

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(New page: A gel run on 6/24)
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[[Image:6.24gel.jpg|700px||center|thumb|A gel run on 6/24]]
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|1. '''Gel Electrophoresis:''' Used this technique to show plasmid DNA sequence. Materials:
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|a. 50 uL of 1% agarose gel
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|b. Buffer TAE
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|c. One gram of 1% agarose per 100 uL of TAE
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|d. Ethidium bromide (intercalating agent)
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|'''Problem encountered:''' electrophoretic gels with 1% agarose had deficient wells
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|'''Solution:''' add 0.5 grams more of agarose to the 100uL of TAE buffer
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[[Image:6.24gel.jpg|700px||center|thumb|Electrophoretic gel run on 6/24]]

Revision as of 15:40, 27 June 2008

1. Gel Electrophoresis: Used this technique to show plasmid DNA sequence. Materials:
a. 50 uL of 1% agarose gel
b. Buffer TAE
c. One gram of 1% agarose per 100 uL of TAE
d. Ethidium bromide (intercalating agent)
Problem encountered: electrophoretic gels with 1% agarose had deficient wells
Solution: add 0.5 grams more of agarose to the 100uL of TAE buffer


Electrophoretic gel run on 6/24
Retrieved from "http://2008.igem.org/Minnesota/24_June_2008"