"
Team:UCSF/Synthetic Chromatin Properties
From 2008.igem.org
| Line 20: | Line 20: | ||
<blockquote> | <blockquote> | ||
<h2 align="justify">Why use Chromatin as a Tool for Synthetic Biology?</h2> | <h2 align="justify">Why use Chromatin as a Tool for Synthetic Biology?</h2> | ||
| - | <p align="justify">Synthetic biologists have traditionally | + | <p align="justify">Synthetic biologists have traditionally employed transcription factors to regulate gene expression. Transcriptional activators and repressors typically work at the level of the promoter, affecting the recruitment of the basal transcription machinery to a specific target gene. The eukaryotic cell, however, has an additional tool to regulate gene expression: heterochromatin (gene silencing).</p> |
<h2 align="justify">Analysis of our Data</h2> | <h2 align="justify">Analysis of our Data</h2> | ||
<p align="justify">Single-cell analysis was done using flow-cytometry. Flow cytometry measurements were taken using a BD LSR-II flow cytometer (BD Biosciences). For each sample, 10,000 cells were counted, and GFP fluorescence was measured by exciting at 488 nm with a 100 mW Coherent Sapphire laser. Cells that were positive for GFP are seen in the microscope images on the lower right panel and their predicted population distribution is shown in the green curve in the ficticious graph in the left (below).</p> | <p align="justify">Single-cell analysis was done using flow-cytometry. Flow cytometry measurements were taken using a BD LSR-II flow cytometer (BD Biosciences). For each sample, 10,000 cells were counted, and GFP fluorescence was measured by exciting at 488 nm with a 100 mW Coherent Sapphire laser. Cells that were positive for GFP are seen in the microscope images on the lower right panel and their predicted population distribution is shown in the green curve in the ficticious graph in the left (below).</p> | ||
Revision as of 23:20, 29 October 2008
Design of our System (previous)
Synthetic Chromatin Bit (Part II)
Why use Chromatin as a Tool for Synthetic Biology?
Synthetic biologists have traditionally employed transcription factors to regulate gene expression. Transcriptional activators and repressors typically work at the level of the promoter, affecting the recruitment of the basal transcription machinery to a specific target gene. The eukaryotic cell, however, has an additional tool to regulate gene expression: heterochromatin (gene silencing).
Analysis of our Data
Single-cell analysis was done using flow-cytometry. Flow cytometry measurements were taken using a BD LSR-II flow cytometer (BD Biosciences). For each sample, 10,000 cells were counted, and GFP fluorescence was measured by exciting at 488 nm with a 100 mW Coherent Sapphire laser. Cells that were positive for GFP are seen in the microscope images on the lower right panel and their predicted population distribution is shown in the green curve in the ficticious graph in the left (below).
The Properties of Our Synthetic Chromatin Bit
We studied how our yeast synthetic chromatin system behaves...
1. Targeting of Sir2 leads to complete silencing of reporter
text here
RESULT 1:
Similar results were obtained for other promoters (e.g. Fig1 P, see below).
2. Dominant over Transcription Factors
text here
RESULT 2:
text here
3. Regional Silencing
text here
RESULT 3:
text
4. Binary
text here
RESULT 4:
text here
text here
5. Memory
text here
RESULT 5:
text here
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Human Practices | Notebooks |
|---|
