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Team:Chiba/Calendar-Home/26 September 2008
From 2008.igem.org
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<br>TIME DELAY TEST | <br>TIME DELAY TEST | ||
| - | <br>colony PCR | + | <br>colony [[Team:Chiba/protocol/PCR|PCR]] |
| - | <br> | + | <br>Vector:[http://partsregistry.org/Part:BBa_R0062 BBa_R0062] Insert:[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]+[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]×7 |
| - | + | ||
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
<tr> | <tr> | ||
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<br>->95°C 5 min ->(95°C 1 min 50°C 30 sec 72°C 1 min)×25 ->72°C 10 min | <br>->95°C 5 min ->(95°C 1 min 50°C 30 sec 72°C 1 min)×25 ->72°C 10 min | ||
| - | <br>->Gel check | + | <br>->[[Team:Chiba/protocol/gelcheck|Gel check]] |
| - | <br>->MINI Prep | + | <br>->[[Team:Chiba/protocol/DNA Purification/sigma|MINI Prep]] |
Revision as of 23:41, 29 October 2008
25 September 2008 <|> 27 September 2008
Laboratory work
Team:Communication
Team:Output
TIME DELAY TEST
colony PCR
Vector:[http://partsregistry.org/Part:BBa_R0062 BBa_R0062] Insert:[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]+[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]×7
| Sample No. | each |
| culture(μL) | 1 |
| Fwd primer(μL) | 1.5 |
| Rev primer(μL) | 1.5 |
| Taq(DNA pol)(μL) | 0.2 |
| dNTPmix(μL) | 3 |
| thermo pol Buffer(μL) | 3 |
| dH2O(μL) | 19.8 |
| TOTAL(μL) | 30 |
->95°C 5 min ->(95°C 1 min 50°C 30 sec 72°C 1 min)×25 ->72°C 10 min
->Gel check
->MINI Prep
