Team:Chiba/Calendar-Home/8 September 2008

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Latest revision as of 23:43, 29 October 2008

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7 September 2008 <|> 9 September 2008

Contents

1 Laboratory work

Laboratory work

Team:Input

no work

Team:Communication

-->(7/9)

Gel Extract

Sample DNA	60
Loading Dye	12
TOTAL	72μL

From left;

[http://partsregistry.org/Part:BBa_ BBa_](2007)

100 ng / 1 well

Sample Name	[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]	[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
Sample DNA	30	60
Loading Dye	6	12
TOTAL	36μL	72μL

From left;

[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]

[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]

Sample DNA	100
Loading Dye	20
TOTAL	6μL

[http://partsregistry.org/Part:BBa_C0261 BBa_C0261] -> none

--->Zymo Clean

eluted with 15μL(?) of NFW.

Team:Output

[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1)
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5)

Sample No.	1	5
DNA(digested sample)(μL)	7	6
SAP(μL)	2	2
SAP Buffer(μL)	3	3
TOTAL(μL)	30	30

-->left at 37 degrees for 1 hour.

-->left at 65 degrees for 30 minutes.

-->DNA purification(1:21μl,5:6μl)

Sample No.	each
DNA(digested sample)(μL)	1
Loading Dye(μL)	1
dH₂O(μL)	4
TOTAL(μL)	6

vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1) 75ng/μl
insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2) 33.6ng/μl
insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](3) 33.6ng/μl
insert:[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](4) 38.4ng/μl
vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) 100ng/μl
insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](6) 33.6ng/μl

Sample No.	1&2	1&3	1&4	1	5&6	5
DNA(vector)(μL)	3	3	3	3	5	2.1
DNA(insert)(μL)	3	3	3	-	3.1	-
Ligase(μL)	1	1	1	1	1	1
Ligase Buffer(x5)(μL)	2	2	2	2	2	2
dH₂O(μL)	1	1	1	4	8.9	4
TOTAL(μL)	10	10	10	10	20	10

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