Team:University of Washington/Protocols

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(New page: == Sequencing == ·primer 8pmol<br\> ·4ul DNA == Mini == ''Qiagen Kit''<br\> 1. 250μL P1 resuspend pellet<br\> 2. 250μL P2<br\> 3. 350μL N3<br\> 4. Centrifuge full speed 10 min.<br\...)
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Revision as of 21:27, 27 June 2008

Sequencing

·primer 8pmol
·4ul DNA

Mini

Qiagen Kit
1. 250μL P1 resuspend pellet
2. 250μL P2
3. 350μL N3
4. Centrifuge full speed 10 min.
5. Decant supernatant into filter cartridge
6. Spin 1 min.

 ·empty collection tube

7. Add 750μL PE wash buffer
8. Spin 1 min.

 ·empty collection tube

9. Dry spin 2 min.
10. Put filter cartridge in clean eppendorf tube
11. Add 50μL EB

 ·Centrifuge 2 min. @ 9000 rpm

[Team:University_of_Washington]

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