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Template:Team:UC Berkeley/Notebook/CDB construction
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PCR ca8373/ca8374 on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) | PCR ca8373/ca8374 on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) | ||
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<pre> | <pre> | ||
| - | Biobricking of promoter for grpE protein | + | Biobricking of promoter for grpE protein on MG1655 |
| - | PCR grpE-F and grpE-R | + | PCR grpE-F and grpE-R (129 bp, EcoR1/BamH1/Dpn1) |
| - | Sub into pBca1256 ( | + | Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) |
Product is pBca1256-K112400 | Product is pBca1256-K112400 | ||
---------------------------- | ---------------------------- | ||
| - | grpE-F Forward Biobricking | + | grpE-F Forward Biobricking ccataGAATTCatgAGATCTccgaggtccttgttgcgaaga |
| - | grpE-R Reverse Biobricking | + | grpE-R Reverse Biobricking cgttaGGATCCcgcattcatctcgctaacttc |
| + | |||
</pre> | </pre> | ||
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<pre> | <pre> | ||
| - | Biobricking of promoter for recA protein | + | Biobricking of promoter for recA protein on MG1655 |
| - | PCR recA-F and recA-R | + | PCR recA-F and recA-R (300 bp, EcoR1/BamH1/Dpn1) |
Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) | Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) | ||
Product is pBca1256-K112401 | Product is pBca1256-K112401 | ||
---------------------------- | ---------------------------- | ||
| - | recA-F Forward Biobricking | + | recA-F Forward Biobricking ccataGAATTCatgAGATCTagagaagcctgtcggcac |
| - | recA-R Reverse Biobricking | + | recA-R Reverse Biobricking cgttaGGATCCcgctttctgtttgtttt |
</pre> | </pre> | ||
==pBca1256-K112402== | ==pBca1256-K112402== | ||
<pre> | <pre> | ||
| - | Biobricking of promoter for fabA protein | + | Biobricking of promoter for fabA protein on MG1655 |
| - | PCR fabA-F and fabA-R | + | PCR fabA-F and fabA-R (279 bp, EcoR1/BamH1/Dpn1) |
Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) | Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) | ||
Product is pBca1256-K112402 | Product is pBca1256-K112402 | ||
---------------------------- | ---------------------------- | ||
| - | fabA-F Forward Biobricking | + | fabA-F Forward Biobricking ccataGAATTCatgAGATCTgccattacgttggctgaa |
| - | fabA-R Reverse Biobricking | + | fabA-R Reverse Biobricking cgttaGGATCCccacccgtttcggtcatt |
</pre> | </pre> | ||
Revision as of 21:30, 27 June 2008
Contents |
PCR ca8373/ca8374 on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112800 ----------------------------------------------- ca8373 Forward Biobricking of mRFP CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag ca8374 Reverse Biobricking of mRFP CGttaGGATCCttactcccccaagaaaatcc
pBca1256-K112400
Biobricking of promoter for grpE protein on MG1655 PCR grpE-F and grpE-R (129 bp, EcoR1/BamH1/Dpn1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112400 ---------------------------- grpE-F Forward Biobricking ccataGAATTCatgAGATCTccgaggtccttgttgcgaaga grpE-R Reverse Biobricking cgttaGGATCCcgcattcatctcgctaacttc
pBca1256-K112401
Biobricking of promoter for recA protein on MG1655 PCR recA-F and recA-R (300 bp, EcoR1/BamH1/Dpn1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112401 ---------------------------- recA-F Forward Biobricking ccataGAATTCatgAGATCTagagaagcctgtcggcac recA-R Reverse Biobricking cgttaGGATCCcgctttctgtttgtttt
pBca1256-K112402
Biobricking of promoter for fabA protein on MG1655 PCR fabA-F and fabA-R (279 bp, EcoR1/BamH1/Dpn1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112402 ---------------------------- fabA-F Forward Biobricking ccataGAATTCatgAGATCTgccattacgttggctgaa fabA-R Reverse Biobricking cgttaGGATCCccacccgtttcggtcatt
