Template:Team:UC Berkeley/Notebook/CDB construction

From 2008.igem.org

(Difference between revisions)
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__toc__
__toc__
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<pre>
 
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PCR ca8373/ca8374 on pSB1A2-I13521    (1261bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112800
 
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-----------------------------------------------
 
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ca8373 Forward Biobricking of mRFP
 
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CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag
 
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ca8374 Reverse Biobricking of mRFP
 
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CGttaGGATCCttactcccccaagaaaatcc
 
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</pre>
 
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==pBca1256-K112400==
==pBca1256-K112400==
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  Product is pBca1256-K112400
  Product is pBca1256-K112400
  ----------------------------
  ----------------------------
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grpE-F Forward Biobricking    cgataGAATTCatgAGATCTccgaggtccttgttgcgaag
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cb0001 grpE-F Forward Biobricking    cgataGAATTCatgAGATCTccgaggtccttgttgcgaag
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grpE-R Reverse Biobricking    cgtagGGATCCcgcattcatctcgctaacttc
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cb0002 grpE-R Reverse Biobricking    cgtagGGATCCcgcattcatctcgctaacttc
</pre>
</pre>
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  Product is pBca1256-K112401
  Product is pBca1256-K112401
  ----------------------------
  ----------------------------
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recA-F Forward Biobricking    cgataGAATTCatgAGATCTagagaagcctgtcggcaccg
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cb0003 recA-F Forward Biobricking    cgataGAATTCatgAGATCTagagaagcctgtcggcaccg
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recA-R Reverse Biobricking    cgtagGGATCCcccagtgctgccgccaacgc
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cb0004 recA-R Reverse Biobricking    cgtagGGATCCcccagtgctgccgccaacgc
</pre>
</pre>
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  Product is pBca1256-K112402
  Product is pBca1256-K112402
  ----------------------------
  ----------------------------
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fabA-F Forward Biobricking  cgataGAATTCatgAGATCTggccattacgttggctgaac  
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cb0005 fabA-F Forward Biobricking  cgataGAATTCatgAGATCTggccattacgttggctgaac  
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fabA-R Reverse Biobricking  cgtagGGATCCgaagttaccacccgtttcgg
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cb0006 fabA-R Reverse Biobricking  cgtagGGATCCgaagttaccacccgtttcgg
</pre>
</pre>

Revision as of 22:01, 27 June 2008

Contents

pBca1256-K112400

Biobricking of promoter for grpE protein 
 PCR grpE-F and grpE-R on MG1655 	  (129 bp, EcoRI/BamHI)
 Sub into pBca1256	                  (EcoRI/BamHI 910+2472bp, L)
 Product is pBca1256-K112400
 ----------------------------
cb0001 grpE-F Forward Biobricking    cgataGAATTCatgAGATCTccgaggtccttgttgcgaag
cb0002 grpE-R Reverse Biobricking    cgtagGGATCCcgcattcatctcgctaacttc

pBca1256-K112401

Biobricking of promoter for recA protein 
 PCR recA-F and recA-R on MG1655 	  (317 bp, EcoR1/BamH1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112401
 ----------------------------
cb0003 recA-F Forward Biobricking    cgataGAATTCatgAGATCTagagaagcctgtcggcaccg
cb0004 recA-R Reverse Biobricking    cgtagGGATCCcccagtgctgccgccaacgc

pBca1256-K112402

Biobricking of promoter for fabA protein 
 PCR fabA-F and fabA-R on MG1655 	  (285 bp, EcoR1/BamH1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112402
 ----------------------------
cb0005 fabA-F Forward Biobricking   cgataGAATTCatgAGATCTggccattacgttggctgaac 
cb0006 fabA-R Reverse Biobricking   cgtagGGATCCgaagttaccacccgtttcgg
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