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User:University of Washington/27 June 2008
From 2008.igem.org
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[[UW Glycerol Stocks]] | [[UW Glycerol Stocks]] | ||
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| + | == LuxR from AraC and TetR == | ||
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| + | - Made glycerol stock | ||
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| + | - Did mini Prep | ||
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| + | - Submitted DNA for sequencing reaction and analysis (only for AraC) | ||
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== == | == == | ||
[[Team:University_of_Washington/Notebook#Notebook]] | [[Team:University_of_Washington/Notebook#Notebook]] | ||
Revision as of 22:17, 27 June 2008
Contents |
Yeast Shuttle Plasmid
- 1.5 mL 15% glycerol stocks of the overnight BY4741 yeast growth cultures were made by combining 563 uL of 40% glycerol and 937 uL yeast culture, then stored at -80 degrees Celsius.
Conjugation
Mini Prep
·R0042 (LuxR promoter)
Glycerol
·R0042 (LuxR promoter)
LuxR from AraC and TetR
- Made glycerol stock
- Did mini Prep
- Submitted DNA for sequencing reaction and analysis (only for AraC)
Team:University_of_Washington/Notebook#Notebook
