Team:Chiba/Calendar-Home/26 September 2008

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===Team:Output===
===Team:Output===
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<br>TIME DELAY TEST
 
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<br>colony [[Team:Chiba/protocol/PCR|PCR]]
<br>colony [[Team:Chiba/protocol/PCR|PCR]]
<br>Vector:[http://partsregistry.org/Part:BBa_R0062 BBa_R0062] Insert:[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]+[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]&times;7
<br>Vector:[http://partsregistry.org/Part:BBa_R0062 BBa_R0062] Insert:[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]+[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]&times;7

Latest revision as of 01:08, 30 October 2008

>Home | Notebook

25 September 2008 <|> 27 September 2008

Laboratory work

Team:Communication

Team:Output


colony PCR
Vector:[http://partsregistry.org/Part:BBa_R0062 BBa_R0062] Insert:[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]+[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]×7

Sample No. each
culture(μL) 1
Fwd primer(μL) 1.5
Rev primer(μL) 1.5
Taq(DNA pol)(μL) 0.2
dNTPmix(μL) 3
thermo pol Buffer(μL) 3
dH2O(μL) 19.8
TOTAL(μL) 30


->95°C 5 min ->(95°C 1 min 50°C 30 sec 72°C 1 min)×25 ->72°C 10 min
->Gel check
->MINI Prep