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• 01:13, 30 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Switch Circuit ‎
• 01:11, 30 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Switch Circuit ‎
• 01:07, 30 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Switch Circuit ‎
• 01:05, 30 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Switch Circuit ‎
• 01:03, 30 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Switch Circuit ‎
• 01:02, 30 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Switch Circuit ‎
• 01:01, 30 October 2008 (diff | hist) N File:Jr rmf 4.jpg ‎ (A Schematic representation of vector pET28a. Not that the vector has a constitutive LacI expression cassette. B BL21 DE3 cells holding the empty pET28a vector at 10mM IPTG. The vector is obviously nontoxic to cells even at high concentrations of inducer. ) (top)
• 01:00, 30 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Switch Circuit ‎
• 00:51, 30 October 2008 (diff | hist) File:Jr gfpsubclone 1.jpg ‎ (uploaded a new version of "Image:Jr gfpsubclone 1.jpg") (top)
• 00:51, 30 October 2008 (diff | hist) File:Jr rmf 3.jpg ‎ (uploaded a new version of "Image:Jr rmf 3.jpg") (top)
• 00:49, 30 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Switch Circuit ‎
• 00:48, 30 October 2008 (diff | hist) N File:Jr pulsegen 5.jpg ‎ (Pulse generator construct [http://partsregistry.org/wiki/index.php?title=Part:BBa_K142027 K142027] was produced from device [http://partsregistry.org/wiki/index.php?title=Part:BBa_K142047 K142047] using PCR-based site-directed mutagenesis as described pr) (top)
• 18:58, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Switch Circuit ‎
• 18:57, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Switch Circuit ‎
• 18:50, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Genome Reduction ‎
• 18:46, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Overview ‎ (→2. Chemostat selection)
• 18:45, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Overview ‎ (→1. Genome Reduction)
• 18:43, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Overview ‎ (→Overview)
• 18:38, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Overview ‎ (→Overview)
• 18:29, 27 October 2008 (diff | hist) Team:Waterloo/Project ‎
• 18:29, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Overview ‎
• 18:15, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Overview ‎
• 18:14, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Overview ‎
• 18:14, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Overview ‎
• 18:13, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Overview ‎
• 18:10, 27 October 2008 (diff | hist) Team:ETH Zurich/Modeling/Overview ‎ (→Overview on the modelling framework)
• 18:10, 27 October 2008 (diff | hist) m Team:ETH Zurich/Wetlab/Overview ‎
• 18:09, 27 October 2008 (diff | hist) Team:ETH Zurich/Modeling/Overview ‎ (→Overview on the modelling framework)
• 18:08, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Overview ‎
• 18:08, 27 October 2008 (diff | hist) m Team:ETH Zurich/Wetlab/Overview ‎
• 18:07, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Overview ‎
• 18:06, 27 October 2008 (diff | hist) Team:ETH Zurich/Modeling/Overview ‎ (→Overview on the modelling framework)
• 18:00, 27 October 2008 (diff | hist) Team:ETH Zurich/Modeling/Overview ‎ (→Overview on the modelling framework)
• 17:57, 27 October 2008 (diff | hist) Team:ETH Zurich/Modeling/Overview ‎ (→Overview on the modelling framework)
• 17:38, 27 October 2008 (diff | hist) Team:ETH Zurich/Team/Overview ‎ (→Lab Course at CNB)
• 17:19, 27 October 2008 (diff | hist) m Team:ETH Zurich ‎
• 16:56, 27 October 2008 (diff | hist) m Team:ETH Zurich ‎ (→Site Map)
• 14:25, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Switch Circuit ‎
• 13:09, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Switch Circuit ‎ (→Fluorescent protein experiments)
• 13:07, 27 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Switch Circuit ‎
• 13:07, 27 October 2008 (diff | hist) N File:Jr pulsegen 4.jpg ‎ (1% agarose gel of PCR products of site directed mutagenesis of the final pulse generator assembly consisting of [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:BBa_I763026 I763026] [http://partsregistry.org/wiki/index.php?ti) (top)
• 12:19, 27 October 2008 (diff | hist) N File:Jr gfpsubclone 1.jpg ‎ (1% agarose gel of XbaI/PstI test restriction digests of plasmid [http://partsregistry.org/Part:pSB3K3 pSB3K3] holding fluorescent protein generators [http://partsregistry.org/Part:BBa_I763004 I763004], [http://partsregistry.org/Part:BBa_J04430 J04430], [h)
• 11:49, 27 October 2008 (diff | hist) N File:Jr pulsegen 3.jpg ‎ (A 1% agarose gel of XbaI/PstI test digests of plasmids holding the lac generator behind the tet-controlled promotor (RL1, RL2, RL3) and of XbaI/PstI test digests of plasmids holding the constitutive tetR expression cassette behind the promotorless lacI ge) (top)
• 11:48, 27 October 2008 (diff | hist) N File:Jr pulsegen 2.jpg ‎ (A 1% agarose gel of PCR products of site directed mutagenesis of C0012. Template DNA of BioBrick [http://partsregistry.org/Part:BBa_C0012 C0012] was amplified by PCR in eight different reactions using primers 276F forward 5’-GGATACGACGATTTTGAAGACAGCTC; ) (top)
• 23:42, 26 October 2008 (diff | hist) N File:Jr rmf 3.jpg ‎ (Cell viability at increasing IPTG concentration. BL21 DE3 cells holding the pET28a-RMF plasmid with mutations R197A; R197F; T276A; T276F; R197A T276A; R197A T276F; R197F T276A; R197F T276F were plated on LB-agar with kanamycin without IPTG, with 1mM IPTG)
• 23:41, 26 October 2008 (diff | hist) N File:Jr rmf 2.jpg ‎ (‘’’A’’’ Growth curve of BL21 DE3 cells holding the pET28a-RMF plasmid without induction (uninduced) and after induction after 5 hours. Cells were grown in 1l of medium in 5l baffled flasks (37C, 95rpm) and induced once they had reached the exp) (top)
• 23:40, 26 October 2008 (diff | hist) N File:Jr rmf 1.jpg ‎ (‘’’A’’’ 1.5% agarose gel with marker 100bp DNA ladder plus (Fermentas); pSB1A7: vector pSB1A7 digested with XbaI and SpeI according to http://www.neb.com NEB manual; RMF: PCR product of RMF amplified from DH5-alpha genomic DNA using forwar) (top)
• 14:51, 26 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Switch Circuit ‎ (→LacIS-mutant)
• 14:51, 26 October 2008 (diff | hist) Team:ETH Zurich/Wetlab/Switch Circuit ‎ (→LacIS-mutant)
• 14:45, 26 October 2008 (diff | hist) N File:Jr pulsegen 1.jpg ‎ (A Lac repressor tetramer, residues R197 and T276 are shown in red. B IPTG bound to the inducer binding site of the lac repressor, residues R197 and T276 are shown in green. Molecular graphics was generated from coordinate set [http://www.rcsb.org/pdb/expl) (top)

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