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User contributions
From 2008.igem.org
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- 21:06, 7 October 2008 (diff | hist) Utah State/6 October 2008 (top)
- 21:04, 7 October 2008 (diff | hist) N Utah State/7 October 2008 (New page: Liquid cultures were made from yesterday's colonies. Transformation will be done tomorrow. Trent) (top)
- 23:55, 6 October 2008 (diff | hist) N Utah State/3 October 2008 (New page: Ligation attempts were made for E0240 into pSB3K3.) (top)
- 23:54, 6 October 2008 (diff | hist) Utah State/6 October 2008
- 23:54, 6 October 2008 (diff | hist) N Utah State/6 October 2008 (New page: Ligations were attempted today for the phbA and phbB genes into the vectors pSB3K3 and pSB1A3. Transformations will be attempted Wednesday. Also, several ligations produced colonies from...)
- 19:15, 22 September 2008 (diff | hist) N Utah State/19 September 2008 (New page: Extracted various promoter regions from electrophoresis gel with Qiagen kit, namely C for-B rev, 5'Cpro for-ATG rev, 5'Cpro for-SD rev, 5'Cpro for-TC rev, -35Cpro for-ATG rev, and -35 pha ...) (top)
- 19:06, 22 September 2008 (diff | hist) N Utah State/22 September 2008 (New page: Isolated plasmid DNA from I20260 and E0240 constructs.) (top)
- 20:30, 19 September 2008 (diff | hist) File:Logo.jpg (uploaded a new version of "Image:Logo.jpg": Reverted to version as of 21:51, 1 July 2008)
- 23:50, 3 September 2008 (diff | hist) N Utah State/3 September 2008 (New page: Linear DNA was extracted from electrophoresis gels for the following constructs: 1. phbA with prefix and suffix 2. phbB with p/s 3. -35 pro with p/s 4. -35/TC with p/s 5. phbB w/out ...) (top)
- 22:40, 27 August 2008 (diff | hist) N Utah State/27 August 2008 (New page: Restriction digestion and electrophoresis was performed for the following: With EcoR1 and Xba1: E0240, E0840, E0420, E0422, E0430, E0240 With Spe1 and Pst1: I712074, R0010, R0040) (top)
- 21:32, 15 August 2008 (diff | hist) N Utah State/15 August 2008 (New page: PCR was run for 16s primers for the H16 cells and M. KMS cells, and Xba1 and Spe1 digestion was performed on portions of all purified DNA; electrophoresis was run for all of these.) (top)
- 21:34, 14 August 2008 (diff | hist) N Utah State/14 August 2008 (New page: DNA isolation was performed for the nine constructs that had not grown before. Plans were made for performing PCR with new primers tomorrow.) (top)
- 21:32, 14 August 2008 (diff | hist) N Utah State/13 August 2008 (New page: Cultures were prepared for the nine cultures that did not grow.) (top)
- 21:31, 14 August 2008 (diff | hist) N Utah State/12 August 2008 (New page: DNA isolation was performed for several constructs, but cultures did not grow for nine of the constructs.) (top)
- 21:30, 14 August 2008 (diff | hist) N Utah State/11 August 2008 (New page: Students learned how to use the DNA isolation kit. Cultures were prepared for all 22 transformed constructs for DNA isolation tomorrow.) (top)
- 20:48, 6 August 2008 (diff | hist) N Utah State/6 August 2008 (New page: Due to negative PCR results, it was decided to re-inoculate H16 Ralstonia Eutropha cultures since there may have been contamination in the culture used during the past two days of PCR. Si...) (top)
- 20:45, 6 August 2008 (diff | hist) N Utah State/5 August 2008 (New page: PCR was again attempted with the same oligos, but the annealing stage was performed on a gradient in the thermocycler. The gradient ranged from 40 to 60 C. Electrophoresis showed negativ...) (top)
- 20:43, 6 August 2008 (diff | hist) N Utah State/4 August 2008 (New page: PCR was done for the phb A,B, and C genes individually and as a whole. Amplification of the various regions of the promoter was also attempted. Electrophoresis showed negative results ex...) (top)
- 19:50, 23 July 2008 (diff | hist) N Utah State/22 July 2008 (New page: We discussed the process of making primers to PCR out genes of interest and how to submit BioBricks. We also looked at Biology Workbench and NCBI to learn the process of analyzing gene se...) (top)
- 05:49, 22 July 2008 (diff | hist) Utah State/18 July 2008 (top)
- 01:58, 19 July 2008 (diff | hist) N Utah State/18 July 2008 (New page: DNA separation preparation was done using the Mini-Scale Preparation for DNA Sequencing by DTAB-DNA Precipitation protocol. Restriction enzyme digestion was done using Xba1 and Pst1. Ele...)
- 21:06, 17 July 2008 (diff | hist) N Utah State/17 July 2008 (New page: Mini-scale preparation for DNA sequencing by CTAB-DNA precipitation was performed for all constructs except I20260 (the full GFP construct sent later by iGEM) up to the point of pellet rem...) (top)
- 21:24, 16 July 2008 (diff | hist) Utah State/16 July 2008 (top)
- 21:22, 16 July 2008 (diff | hist) N Utah State/16 July 2008 (New page: The I20260 construct was mistakenly placed on Amp plates. The transformation was redone today and placed on Kan plates. At least one colony per construct was noted (excepting I20260) and...)
- 16:55, 15 July 2008 (diff | hist) N Utah State/15 July 2008 (New page: None of the eight transformations from yesterday worked. An ampicillin plate from the batch we made yesterday was streaked with previously successfully transformed with T7 luciferase E. c...) (top)
- 16:47, 15 July 2008 (diff | hist) N Utah State/14 July 2008 (New page: Today we attempted transformations for three promoters (T7-I712074, lac-R0010, and the CFP/RFP-R0040 promoters) and five promoterless reporters (GPF-E0240 and E0840, ECFP-E0420 and E0422, ...) (top)
- 16:27, 10 July 2008 (diff | hist) Utah State/10 July 2008 (top)
- 16:25, 10 July 2008 (diff | hist) N Utah State/10 July 2008 (New page: The fluorescence of the GFP needing IPTG was tested. The sample without IPTG had an OD of 0.9 and the sample with IPTG had an OD of 0.7, not subtracting the media control. A fluorescence...)
- 20:41, 9 July 2008 (diff | hist) N Utah State/8 July 2008 (New page: Restriction enzyme digest preparations were performed in order to do the digestion tomorrow for the three GFP strengths, the GFP needing IPTG to fluoresce, and the lux construct.) (top)
- 20:38, 9 July 2008 (diff | hist) N Utah State/9 July 2008 (New page: A restriction enzyme digest was performed with the three GFP constructs, the luciferase construct, and the GFP (needing IPTG). An electrophoresis gel was run. Colonies were prepared for ...) (top)
- 17:29, 3 July 2008 (diff | hist) N Utah State/3 July 2008 (New page: Fluorescence was tested for all of the GFP constructs. A notable difference was noted between the weak, medium, and strong promoter constructs. Excitation was 488 nm and emission tested ...) (top)
- 17:25, 3 July 2008 (diff | hist) N Utah State/2 July 2008 (New page: Colonies appeared for all transformations from yesterday. Liquid and streak plate cultures were created from these.) (top)
- 21:56, 1 July 2008 (diff | hist) N Utah State/1 July 2008 (New page: We retransformed the three GFP constructs sent to us from iGEM, namely the weak, medium, and strong promoter GFPs. We also transformed the GPF that was noted to work well after adding ITP...) (top)
- 21:51, 1 July 2008 (diff | hist) N File:Logo.jpg
- 21:51, 1 July 2008 (diff | hist) Team:Utah State
- 20:30, 1 July 2008 (diff | hist) N File:Team logo.jpg
- 20:30, 1 July 2008 (diff | hist) File:Example logo.png
- 18:23, 1 July 2008 (diff | hist) Team:Utah State
- 18:22, 1 July 2008 (diff | hist) N File:Main Picture 2.jpg (top)
- 18:20, 1 July 2008 (diff | hist) Team:Utah State
- 17:58, 1 July 2008 (diff | hist) N File:Main Picture.jpg (top)
- 17:57, 1 July 2008 (diff | hist) Team:Utah State
- 17:48, 1 July 2008 (diff | hist) Team:Utah State
- 17:47, 1 July 2008 (diff | hist) Team:Utah State
- 17:35, 1 July 2008 (diff | hist) N File:Puching out genes.JPG (top)
- 17:50, 20 June 2008 (diff | hist) N Utah State/20 June 2008 (New page: Today we looked at the electrophoresis results from yesterday's pictures. The RFP, Banana, Wintergreen, and CFP DNA runs were as expected and are indicative of successful transformations....) (top)
- 18:11, 18 June 2008 (diff | hist) N Utah State/17 June 2008 (New page: We learned a good amount about how to use the Spectrofluorometer today by finding optimal Excitation and Emission wavelengths for the four fluorescent proteins we have transformed into E. ...) (top)
- 19:00, 9 June 2008 (diff | hist) Utah State/9 June 2008 (top)
- 18:57, 9 June 2008 (diff | hist) N Utah State/9 June 2008 (New page: Two agar streak plates and two 3-ml liquid cultures were prepared from two colonies of the Wintergreen scent, Banana scent, and Salicylate Generator plates and placed in the incubator. St...)
- 18:51, 9 June 2008 (diff | hist) N Utah State/6 June 2008 (New page: Colonies were seen on the (ampicillin) plates for the wintergreen scent, banana scent, and salicylate generator plates, but not for the Lux ABCDE and T7 Promoter plates, which were left in...) (top)
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