Team:University of Ottawa/30 June 2008

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Today in the lab

Dan

Gel of friday-saturday ligation was run
  • Ligation bands are very faint
  • Decided to perfrom PCR cleanup on reaction mixture
  • This time I used the correct amount all reactants
  • Still, gel indicated no ligation after an hour
  • WIll leave in PCR machine and check tomorrow
    • Digestion of 0
  • Plasmid 0 containing our receptor was digested overnight with EagI in order to remove GFP which is already in the plasmid and will interfere with our system.
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/30_June_2008"