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Template:Team:UC Berkeley/Notebook/SC construction
From 2008.igem.org
Contents |
pBca1256-K112600
Construction of <AP-tag> basic part K112600 Wobble PCR SC001/SC002 (66 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112600 ---------------------------------------------------- SC001F Forward Biobricking of <AP-tag> cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag SC002R Reverse Biobricking of <AP-tag> ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg
pBca1256-K112601
Construction of <AP-tag! basic part K112601 Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112601 -------------------------------------------------- SC001 Forward Biobricking of <AP-tag! cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag SC003 Reverse Biobricking of <AP-tag! ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg
pBca1256-K112602
Construction of <FLAG-tag> basic part K112602 Wobble PCR SC004/SC005 (45 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112602 ------------------------------------------------------------- SC004 Forward Biobricking of <FLAG-tag> cgataGAATTCatgAGATCTgactacaaggatgacgacg SC005 Reverse Biobricking of <FLAG-tag> ccagtGGATCCcttgtcgtcgtcatccttgtagtc
pBca1256-K112603
Construction of <FLAG-tag! basic part K112603 Wobble PCR SC004/SC006 (48 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112603 ------------------------------------------------------------- SC004 Forward Biobricking of <FLAG-tag! cgataGAATTCatgAGATCTgactacaaggatgacgacg SC006 Reverse Biobricking of <FLAG-tag! ccagtGGATCCttacttgtcgtcgtcatccttgtagtc
pBca1256-K112604 (M)
Construction of {rbs_barstar>} basic part K112604 (320 bp, EcoRI/BamHI)
PCR sc007/sc008 on Bacillus amyloliquefaciens gen. (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112604
-------------------------------
sc007 Fwd biobricking of {rbs_barstar>}
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
sc008 Reverse biobricking of {rbs_barstar>}
cgttaGGATCCagaaagtatgatggtgatgtcgc
pBca1256-K112605 (E)
Construction of {prepro>} of PhoA basic part K112605 (114 bp, EcoRI/BamHI)
CR sc009/sc010 on MG1655 (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112605
-------------------------------------------------------------
sc009 Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTgtacatggagaaaataaaatgaaacaaagc
sc010 Reverse biobricking of {prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
pBca1256-K112606 (E)
Construction of {prepro>} of LamB basic part K112606 (132 bp, EcoRI/BamHI)
PCR sc011/sc012 on MG1655 (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112606
-------------------------------------------------------------
sc011 Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTgaaaagcaatgactcaggagatag
sc012 Reverse biobricking of {prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
pBca1256-K112607
Construction of {a~prepro>} of PhoA basic part K112607 (100 bp, EcoRI/BamHI)
PCR sc013/sc010 (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112607
------------------------------------------------------
sc013 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCT taaa Atgaaacaaagcactattgcac
sc010 Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
pBca1256-K112608
Construction of {a~prepro>} of LamB basic part K112608 (111 bp, EcoRI/BamHI)
PCR sc014/sc012 (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112608
--------------------------------------------------
sc014 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCT taga atgatgattactctgc
sc012 Reverse biobricking of {a~prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
pBca1256-K112609
Construction of {rbs_prepro>} of PhoA basic part K112609 (113 bp, EcoRI/BamHI)
PCR sc015/sc010 (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112609
------------------------------------------------------
sc015 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCT gtacatggagaaaataaaAtgaaacaaag
sc010 Reverse biobricking of {rbs_prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
pBca1256-K112610
Construction of {rbs_prepro>} of LamB basic part K112610 (126 bp, EcoRI/BamHI)
PCR sc016/sc012 (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112610
------------------------------------------------------
sc016 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCT caatgactcaggagataga atgatgattac
sc012 Reverse biobricking of {rbs_prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
