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Team:University of Ottawa/3 July 2008
From 2008.igem.org
Contents |
Today in the Lab
Matt
- Competent Cells
- Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of growth
- The cells containing the ligation product will then be inoculated and left to incubate overnight for a miniprep tomorrow.
- The Ecoli containing the dehydrogenase component failed to grow from streaking a plate yesterday,I will try and streak again today and then glycerol stock the remaining Ecoli.
- Ligation
- Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again today.
Dan
- Atcre integration
- Plate had ~4 colonies so I decided to redo integration.
- 1,2,3,4 and S,D,T ligation
- The concentration of these constructs was determined to be decent
- A gel was run to verify the integrity of the constructs, S,D,T showed some contamination
- 1,2,3,4 did not show up on the gel for unknown reasons.
Dan and Matt
- Integration
- Atcre, and 1S 1D 1T 2S 2D 2T 3S 3D 3T 4S 4D 4T were integrated
