Team:Johns Hopkins/Protocols
From 2008.igem.org
Welcome to Johns Hopkins iGEM
The International Genetically Engineered Machines Competition (iGEM) is an annual intercollegiate challenge that seeks to answer the question: "Can simple biological systems be built from standard, interchangeable parts and operated in living cells? Or is biology simply too complicated to be engineered in this way?" The newly-formed iGEM team at Johns Hopkins is composed of mostly undergraduate students with diverse majors ranging from Materials Science Engineering to Biology. While the team has graduate and faculty input, it is almost totally undergraduate run. The iGEM teams work over the summer at their, respective university, and then attend the iGEM Jamboree in November to present their work. We plan to create novel genetic parts that could be added to the existing iGEM registry of biological parts. The MIT iGEM Registry of Parts is the databank of biological standards to which iGEM teams from 2003 and onward have contributed. Currently, the databank only has 16 yeast "biobricks". These biobricks are reporters, tags, plasmids and other useful interchangeable genetic parts, that could one day revolutionize genetic and synthetic biology. By the end of our project, we will have most likely doubled the amount of yeast biobricks in the registry.
Ligation and Transformation Protocol
Plasmid Miniprep Protocol