Team:ESBS-Strasbourg/14 July 2008

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Contents

DryLab

Modeling

WetLab

results from the transformation

  • 99 colonies of the control plasmid in TOP10(invitrogen), 5*10^6 cfu/ug (still not enough)
  • 1 colonie of the BBa_J63002(Adh1_ter) in TOP10(invitrogen), PCR with primers <<verification pSB1A3 left/right>> should deliver a 461 bp fragment


  • Media
    • bacteria: LB-medium (500 ml), LB-medium for Ampicillin addition (250 ml), LB-Agar-plates without antibiotics (7)
    • yeast: YPD-medium (500 ml)


  • Overnight cultures
    • bacteria:
    • yeast: strain BYA4742

General

Quote of the day

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