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From 2008.igem.org

E.Coli

Preparation of competent cells (Mariels/Barbaras Protocol) Competent Bacteria

Preparation of TOP10 chemically competent cells [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol]

Transformation of competent cells (Mariels/Barbaras Protocol) Transformation

Cloning PCR Purification of PCR products Miniprep:Plasmid Amplification Plasmid Purification Restriction Digestion Agarose Gel Preparation Dephosphorylation Ligation

Solutions and buffers SOB for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB]

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