Team:ESBS-Strasbourg/16 July 2008

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Contents

DryLab

michi:

  • IRESes s*** let's mutate our promoter
  • last chance for IRES: wrote email to Pospisek
  • Calculated size of our constructs: 6 kb (too big for vector?)

Wiki

  • Further content in all categories (MM)

Modeling

WetLab

Sandra:

  • Gel of the PCR from yesterday
  • Transformations
    • cells | part/vector | protocol

dh5a (Mariel) | pxj (10 ng) | HeatShock (Maria) 

     =       |      =           | KCM (Barbara)
     =       | LexA-BD (1 µl)   |      =
     =       | BB_pSB (2 µl)    |      =
     =       | BB_1763004 (2 µl)|      =

dh5a (ours) | pxj (10 ng) | HS Top10 | = | = db3.1 | = | = 

 =           | BB_p1010 (2 µl)  | =


Mariel repeats the PCR from Paul from yesterday with 10ng plasmids
=>Amplification of Gal4 succeed

Paul:

  • Purification of Gal4 PCR product, stored at -20°C

General

Quote of the day

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