Team:The University of Alberta/21 July 2008

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Today

  • Transformed the ligation of Tryp into J6
  • Finished Westerns of the Butanol suff in J6...RESULTS PENDING
    • Results in. Didn't work. Again. Either we're doing the Westerns incorrectly or the butanol parts aren't being translated for some reason.
  • Worked out the procedure for putting together all of our parts.
  • Re-digesting Lac1 ERE, TetR. RBS.6x His, RBS-6xHis, TDNA-MCS with Xba/Pst.
  • Digested BisDA and BisDB with Xba/Pst. Ran them on gel.
    • They looked good, so gel purified the bands.
  • Cut I0500 with Spe and Pst. No bands appeared on gel. Reran them on gel and got two bands.
    • Set up the O/N's using the colonies on plate. Minipreps to be done on Tuesday.
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