| Digestion Products
|
| Number
| Product Name
| Matrix Name
| Enzyme 1
| Enzyme 2
| Size
| Description
| Product Left
| []ng/µL
| Date
|
| D1
| <bbpart>pJ23100</bbpart>
| <bbpart>pJ23100</bbpart>
| Spe1
| Pst1
| 2096
| Vector with the J23100 promoter ready for insertion of a RBS/CDS insert
| Ø (24/09/07)
| 34
| July 17
|
| D2
| <bbpart>mRFP dblTer</bbpart>
| <bbpart>pJ23100</bbpart>
| Spe1
| Pst1
| 887
|
|
| 27
| July 17
|
| D3
| <bbpart>pJ23107</bbpart>
| <bbpart>pJ23107</bbpart>
| Spe1
| Pst1
| 2096
| Vector with the J23107 promoter ready for insertion of a RBS/CDS insert
| Ø (24/09/07)
| 32
| July 17
|
| D4
| <bbpart>mRFP dblTer</bbpart>
| <bbpart>pJ23107</bbpart>
| Spe1
| Pst1
| 887
|
|
| 25
| July 17
|
| D5
| <bbpart>pJ23100</bbpart>
| <bbpart>pJ23100</bbpart>
| EcoR1
| Spe1
| 2925
| The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
|
| 28&29
| July 17
|
| D6
| <bbpart>pJ23107</bbpart>
| <bbpart>pJ23107</bbpart>
| EcoR1
| Spe1
| 2925
| The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
|
| 31&27
| July 17
|
| D7
| Lox66 DapA E.coli
| Lox66 DapA E.coli (PCR product)
| EcoR1
| Pst1
| 977
|
|
| 24
| July 17
|
| D8
| Lox66 DapA E.coli
| Lox66 DapA E.coli (PCR product)
| Xba1
| Pst1
| 962
|
|
| 44
| July 17
|
| D9
| <bbpart>pJ23100</bbpart> ES vector
| <bbpart>pJ23100</bbpart> (MP1.1 & MP1.2)
| EcoI
| SpeI
| 2925
| Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert)
|
| 80&43
| July 21
|
| D10
| <bbpart>pJ23100</bbpart> promoter fw-insert
| <bbpart>pJ23100</bbpart> (MP1.1 & MP1.2)
| EcoI
| SpeI
| 58
| J23100 promoter ready for use as a fw-insert
|
| 35&28
| July 21
|
| D11
| <bbpart>pJ23107</bbpart> ES vector
| <bbpart>pJ23107</bbpart> (MP2.1, MP2.2, MP2.3 & MP2.4)
| EcoRI
| SpeI
| 2925
| Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter
|
| 49,35,28&41
| July 21
|
| D12
| <bbpart>pJ23107</bbpart> promoter fw-insert
| <bbpart>pJ23107</bbpart> (MP2.1, MP2.2, MP2.3 & MP2.4)
| EcoRI
| SpeI
| 58
| J23107 promoter ready for usage as a fw-insert
|
| 23,25,32&32
| July 21
|
| D13
| <bbpart>mRFP</bbpart>
| <bbpart>pJ23107</bbpart> (MP2.2, MP2.3 & MP2.4)
| SpeI
| PstI
| 887
| mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it
|
| 21&41
| July 21
|
| D14
| Cre ORF
| <bbpart>J61047</bbpart> MP9.1 & MP9.2
| XbaI
| PstI
| 1063
| Cre ORF extracted for usage as a bw-insert
| Ø D14.1
| 38&41
| July 21
|
| D15
| <bbpart>B0030</bbpart> bw-vector
| <bbpart>B0030</bbpart> MP5.1 & MP5.2
| SpeI
| PstI
| 2076
| bacward vector for cloning a CDS bw-insert after the B0030 RBS
|
| 87&42
| July 21
|
| D18
| BB dig. lox66DapAE.coli
| lox66-DapAE.coli P5 PCR product
| EcoRI
| PstI
| ~960
| PCR product digested for cloning as a BioBrick
| Ø
| 44
| July 21
|
| D19
| BB dig. lox66DapAsubtilis
| lox66-DapAsubtilis P6 PCR product
| EcoRI
| PstI
| ~960
| PCR product digested for cloning as a BioBrick
| Ø
|
| July 21
|
| D19'
| BB dig. lox66DapAsubtilis
| lox66-DapAsubtilis P6' PCR product
| EcoRI
| PstI
| ~960
| PCR product digested for cloning as a BioBrick
|
| 16
| July 30
|
| D20
| bw-insert lox66-DapAE.coli
| lox66-DapAE.coli P5 PCR product
| XbaI
| PstI
| ~960
| PCR product digested for usage as a backward insert
| Ø
|
| July 21
|
| D21
| bw-insert lox66-DapAsubtilis
| lox66-DapAsubtilis P6 PCR product
| XbaI
| PstI
| ~960
| PCR product digested for usage as a backward insert
| Ø
|
| July 21
|
| D21'
| bw-insert lox66-DapAsubtilis
| lox66-DapAsubtilis P6' PCR product
| XbaI
| PstI
| ~960
| PCR product digested for cloning as a BI
|
| 34
| July 30
|
| D22
| pSB1A2 open vector
| <bbpart>BBa_J61047</bbpart> (MP9.1 & MP9.2)
| EcoI
| PstI
| Ø D22.1
| Open pSB1A2 vector for BioBrick EcoRI/PstI insertio
| Ø (24/09/07)
| D22.2:59
| July 23, Aug. 13
|
| D23
| AraC/pBad promoter fw-insert
| <bbpart>BBa_I0500</bbpart> (MP4.1 & MP4.2)
| EcoI
| SpeI
| 1233
| AraC/pBad promoter ready for use as a forward insert
| Ø D23.1
| 93&53
| July 23
|
| D24
| eCFP bw-insert
| <bbpart>BBa_E0422</bbpart> (MP6.1 & MP6.2)
| XbaI
| PstI
|
| eCFP (RBS-OFR_LVA-T) ready for use as a backward insert
|
| 56&55
| July 23
|
| D26
| gfp-tripart
| <bbpart>BBa_E0840</bbpart> (MP8.1 & MP8.2)
| XbaI
| PstI
|
| gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert
|
| 47&47
| July 23
|
| D27
| lox71-ftsZ bw-insert
| lox71-ftsZ PCR product P7
| XbaI
| PstI
|
| lox71-ftsZ(unmutated) ready for use as a backward insert
| Ø
|
| July 23
|
| D27'
| lox71-ftsZ bw-insert
| lox71-ftsZ PCR product P7
| XbaI
| PstI
|
| lox71-ftsZ(unmutated) ready for use as a backward insert
|
| 24
| July 30
|
| D28
| RBS-DapAColi BB
| P8 (RBS-DapAColi)
| EcoRI
| PstI
| ~950
| Biobrick
|
| 54
| July 25
|
| D29
| RBS-DapAColi
| P8 (RBS-DapAColi)
| XbaI
| PstI
| ~950
| Backward insert
|
| 44
| July 25
|
| D30
| RBS-DapASubtilis BB
| P9 (RBS-DapASubtilis)
| EcoRI
| PstI
| ~950
| Biobrick
|
| 72
| July 25
|
| D31
| RBS-DapASubtilis
| P9 (RBS-DapASubtilis)
| XbaI
| PstI
| ~950
| Backward insert
|
| 51
| July 25
|
| D32
| Lox71-FtsA-FtsZ
| P10 Lox71-FtsA-FtsZ
| XbaI
| PstI
| ~2600
| Backward insert - EcoRI site still presents : we can't use EcoRI
|
| 47
| July 25
|
| D33
| RBS(B0030)CreORF
| L6' (clone 1 & 2)
| XbaI
| PstI
|
| RBS-Cre digestion for use as a BI
|
| 29&27
| July 30, August 13
|
| D34
| lox66
| L9' lox66
| SpeI
| PstI
|
| Biobrick plasmid carrying lox66 digested as a BV
|
| 18
| July 30
|
| D35
| lox71
| L10' lox71
| SpeI
| PstI
|
| Biobrick plasmid carrying lox71 digested as a BV
|
|
| July 30, August 13
|
| D36
| lox71-KmR-lox66
| P11 lox71-KmR-lox66
| EcoRI
| PstI
|
| lox71-KmR-lox66 digested as a biobrick insert
|
|
| July 30
|
| D37
| lox71-gfp(tripart) BI
| L12.1 & L12.2 miniprep products (from 30/07 ligation reaction)
| XbaI
| PstI
|
| lox71_RBS-gfp-double terminator extracted as a backward insert
|
|
| Aug. 6
|
| D38
| BB-insert lox66-DapAE.coli
| lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2)
| EcoRI
| PstI
|
| lox66-dapAE.coli extracted from pTOPO plasmid as a BB insert
|
|
| Aug. 6
|
| D39'
| BI-insert lox66-DapAE.coli
| lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2)
| XbaI
| PstI
|
| lox66-dapAcoli extracted for usage as a backward insert
|
|
| Aug. 7
|
| D40
| BB-insert lox71-KmR-lox66
| L25.1 miniprep product (from ligation rxn of 30/07
| EcoRI
| PstI
|
| lox71-KmR-lox66 construct extracted for clonong as a biobrick
|
|
| Aug. 6
|
| D41
| araC/pBad-J61002 plasmid SP dig.
| L1.2, L1".1 & L1".2 miniprep product
| SpeI
| PstI
|
| J61002 plasmid containing araC/pBad promotor digested for insertion of a BI CDS
|
|
| Aug. 13
|
| D42
| Lox66 FV
| L9
| EcoRI
| XbaI
|
| pSB1A2-lox66 as a Forward vector
|
|
| August 13
|
| D43
| gfp-tripart FI
| MP8.1
| EcoRI
| SpeI
|
| gfp-tri part (strongRBS-ORF-T) ready for use as a forward insert
|
|
| August 13
|
| D44
| <bbpart>B0015</bbpart> terminator BI
| <bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.3 Aug.14)
| XbaI
| Pst1
|
| terminator B0015 extracted for use as a BI
|
|
| August 16
|
| D45
| <bbpart>B0015</bbpart> terminator FI
| <bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.4 Aug.14)
| EcoRI
| SpeI
|
| terminator B0015 extracted for use as a FI
|
|
| August 16
|
| D46
| <bbpart>B0015</bbpart> BV
| <bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.3 Aug.14)
| SpeI
| Pst1
|
| <bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> plasmid extracted for use as a BV
|
|
| August 16
|
| D47
| <bbpart>pSB1A2</bbpart> XP dig.
| <bbpart>pSB1A2</bbpart> carrying Cre ORF (MP9.1)
| XbaI
| PstI
|
| <bbpart>pSB1A2</bbpart> open vector ready for cloning of BI
|
|
| August 16
|
| D48
| <bbpart>pSB1A2</bbpart> ES dig.
| <bbpart>pSB1A2</bbpart> carrying Cre ORF (MP9.2)
| EcoRI
| SpeI
|
| <bbpart>pSB1A2</bbpart> open vector ready for cloning of FI
|
|
| August 16
|
| D49
| lox71-gfptripart BI
| lox71-gfptripart in <bbpart>pSB1A2</bbpart> (L12.2)
| XbaI
| Pst1
|
| lox71-gfptripart extracted for use as a BI
|
|
| August 16
|
| D50
| <bbpart>BBa_J61002</bbpart> pTet SP dig.
| <bbpart>BBa_J61002</bbpart> (MP13.1 Aug. 14)
| SpeI
| PstI
|
| <bbpart>BBa_J61002</bbpart> pTet promoter vector ready for insertion of CDS as a BI
|
|
| August 16
|
| D51
| RBS-dapAcoli BBinsert
| pTOPO of RBS-dapAcoli (MPT4.3)
| EcoRI
| Pst1
|
| RBS-dapAcoli extracted for cloning as a biobrick
|
|
| August 16
|
| D52
| RBS-dapAcoli BI
| pTOPO of RBS-dapAcoli (MPT4.3)
| XbaI
| Pst1
|
| RBS-dapAcoli extracted for use as a BI
|
|
| August 16
|
| D53
| RBS-dapAsubtilis BBinsert
| pTOPO of RBS-dapAsubtilis (MPT5.1)
| EcoRI
| Pst1
|
| RBS-dapAsubtilis extracted for cloning as a biobrick
|
|
| August 16
|
| D54
| RBS-dapAsubtilis BI
| pTOPO of RBS-dapAsubtilis (MPT5.1)
| XbaI
| Pst1
|
| RBS-dapAsubtilis extracted for use as a BI
| Ø (24/09/07)
|
| August 16
|
| D55
| RBS-dapAsubtilis FI
| pTOPO of RBS-dapAsubtilis (MPT5.2)
| EcoRI
| SpeI
|
| RBS-dapAsubtilis extracted for use as a FI
|
|
| August 16
|
| D57
| lox71-B0015(T) BI
| (L31.1, L31.3) lox71-B0015(T) <bbpart>pSB1A2</bbpart>
| XbaI
| Pst1
|
| lox71-B0015(T) extracted for use as a BI <bbpart>pSB1A2</bbpart>
|
|
| Sep. 14
|
| D58
| B0015(T)-lox66 BV
| (L32.1, L32.2) B0015(T)-lox66 BV
| SpeI
| PstI
|
| B0015(T)-lox66 for use as a BV <bbpart>pSB1A2</bbpart>
|
|
| Sep. 14
|
| D59
| pTet>>lox71-gfptripart BI
| (L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
| XbaI
| PstI
|
| pTet>>lox71-gfptripart for use as a BI
|
|
| Sep. 14
|
| D61
| lox66-mRFP FV
| (L36.1, L36.2) lox66-mRFP
| EcoRI
| XbaI
|
| lox66-mRFP part (strongRBS-ORF-T) ready for use as a forward vector
|
|
| Sep. 14
|
| D62
| lox66-RBSDapAcoli BI
| (L37.1, L37.2) lox66-RBSDapAcoli
| XbaI
| PstI
|
| lox66-RBSDapAcoli ready for use as a Backward insert
|
|
| Sep. 14
|
| D63
| RBSDapAcoli BI
| (L39.1, L39.2) RBSDapAcoli
| XbaI
| PstI
|
| RBSDapAcoli BI (for insertion behind T-lox66)
|
|
| Sep. 14
|
| D64
| FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> BV
| (MP14.1) <bbpart>BBa_J61025</bbpart>
| SpeI
| PstI
|
| FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for backward introduction of a casette for Wanner K insertion)
|
|
| Sep. 14
|
| D65
| FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> FV
| (MP14.1) <bbpart>BBa_J61025</bbpart>
| EcoR
| XbaI
|
| FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for forward introduction of a casette for Wanner K insertion)
|
|
| Sep. 14
|
| D68
| attB BV
| (MP15.1)
| SpeI
| PstI
|
| vector containing attB for use as a BV
|
|
| Sep. 14
|
| D69
| gfptripart-lox66 BV
| (L29.1) gfptripart-lox66 <bbpart>pSB1A2</bbpart>
| SpeI
| PstI
|
|
|
|
| Sep. 14
|
| D70
| pTet>>lox71-gfptripart FI
| (L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
| EcoRI
| SpeI
|
| pTet>>lox71-gfptripart for use as a FI
|
|
| Sep. 14
|
| D71
| araC/pBad>>lox71-gfptripart FI
| L35.1 araC/pBad>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
| EcoRI
| SpeI
|
| araC/pBad>>lox71-gfptripart for use as a FI
| Ø (24/09/07)
|
| Sep. 14 Sep. 17
|
| D72
| <bbpart>pSB2K3</bbpart> BBvector
| MP16 <bbpart>pSB2K3</bbpart>::attP
| EcoRI
| PstI
|
| Low copy number plasmid, BB vector
|
|
| Sep. 17
|
| D73
| <bbpart>pSB2K3</bbpart> ES open vector
| MP16 <bbpart>pSB2K3</bbpart>::attP
| EcoRI
| SpeI
|
| Comparison of digestion efficiency between Pst and Spe in NEB2
|
|
| Sep. 17
|
| D76
| DGAT BB
| P14 DGAT
| EcoRI
| PstI
|
| DGAT
| Ø (24/09/07)
|
| Sep. 17
|
| D77
| araC/pBad>>lox71-gfptripart BI
| D60 (.1 & .2) digestion product
| FspI
| AhdI
|
| in D60, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion
|
|
| Sep. 17
|
| D78
| araC/pBad>>lox71-gfptripart FI
| D71 (.1 & .2) digestion product
| FspI
| AhdI
|
| in D71, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion
|
|
| Sep. 17
|
"
