Team:The University of Alberta/23 May 2008

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Revision as of 20:44, 23 May 2008 by Cwk (Talk | contribs)

Contents

Volunteers Scheduled for Today

5-8 AL
5-8 KR


Today

  • Work on the Purple Russian and The Blue Ox
  • Finish the Binary Vector

The legacy of the Purple Russian and the Blue Ox

The Purple Russian being the purple GFP
The Blue Ox is two enzymes that Mike dropped off that will cause our bacteria to go blue

Today
We recieved the purple russian and blue ox from mike (in pUC57) and will transform them into bacteria to make glycerol stocks. We will then design primers to turn these into biobricks.

What we accomplished today

Chris:

  • Jason and I transformed Purple Russian (ASFSP95_CDS) and Blue Ox (Dioxygenase and Tryp.) into XL10.
  • Plated out the transformants (10ul, 100ul, 400ul per sample) and placed in the incubator. I'll come in tomorrow to take them out and place them in the fridge to make sure they dont end up as a lawn of bacteria when we come in on Monday.
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