Wisconsin: Lignin Project/17 June 2008

Harvested the chromosomal DNA from the overnight MG1655 cultures by boiling the cells.

Used this DNA as a template for 2 PCR reactions

One reaction was to clone out srlD the other was to clone the srl operon.

However when run on a gel no bands were found.

Team Fungus:
Received primers
Diluted primers
Ran TAQ PCR reaction with Carbon-limited cDNA
Ran PCR product on 1% agrose gel; only ladder visible