Team:KULeuven/1 August 2008

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Contents

Lab Work

Wet Lab

  • The ligated plasmids that were transformed into TOP10 cells yesterday, didn't give any colonies. We think that maybe the transfomation is the problem. So we wanted to use electroporation to transform the cells, but we ran out of competent cells.
  • The primers to make GFP with LVA tag arrived today. So we did a PCR to amplify this part and to attach the prefix and suffix. GFP-LVA is now on its way to become a BioBrick (after ligation into a plasmid).

Dry Lab

Modeling

Part one of the mathematical analysis of the memory is done: stability of the two stationary states is proven. For next week: try to find the boundary between the initial states going to one of the two stationary states.

Wiki

Remarks