"
Team:KULeuven/7 August 2008
From 2008.igem.org
Revision as of 17:33, 7 August 2008 by Elkevanassche (Talk | contribs)
| << return to notebook | return to homepage >> | ||
| < previous friday | ← yesterday | tomorrow → | next monday > |
Contents |
Lab Work
Dr. Coli overview (for now)
Wet Lab
- F1610 wasn't cut with XbaI. We are doing this again. The result will follow tomorrow.
- The primers finally arrived and we are doing a PCR on the ligated parts.
- We'll do some ligations today: C0012 + B0015, R0062+B0032, R0084+J23022.
- The cells we transformed yesterday all gave colonies. We streaked them out and made a fluid culture.
- The digestions we did yesterday were purified from gel.
Dry Lab
Cell death contains an error: P1010 is a part which constitutively produces ccdB. It already contains a promoter and a RBS, see the [http://partsregistry.org/Part:BBa_P1010 parts page] and the [http://partsregistry.org/cgi/partsdb/part_info.cgi?part_name=BBa%20P1010 Hard Information]. Primers will have to be made to create a full-blown biobrick with just the ccd locus. A new part ([http://partsregistry.org/Part:BBa_K145151 K145151]) has been made from P1010 with just the ccdB coding sequence. We will PCR this part starting from P1010 with the correct primers.
