Wisconsin: Lignin Project/12 June 2008

We designed and ordered primers for cloning srlD and the srloperon out of E. coli MG1655.

These primers will also add an xba1 site as well as HindIII site to each end of the gene and operon so they can easily be cloned into a plasmid vector.

Note: on the srlD primer we added a Shine-Delgarno sequence so that the gene can be expressed without the operon.

We also chose pBAD30 and pBAD18 as our starting (and storage) plasmid vectors to clone into.

They are both arabanose inducible and low and high copy number (respectively)