Wisconsin: Lignin Project/16 June 2008

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Team Sorbitol:

Harvested the pBAD30 and pBAD18 from the cells using a Qiagen Miniprep Kit.

Stored this DNA in a -20 Freezer

Began cultures of MG1655 to use for cloning.

Experimented with different media recipes for optimal growth.

    M9 minimal media with: 1.) 20%glucose +++, 20% glycerol++, 20%acetate+

Met with Jennie Reed, modeling work adding sorbitol to basic cell model

Team Fungus:
Made LB + Kanamycin plates
Streaked out vector (pet28a) on LB + Kanamycin plates
Made dilute Kanamycin freezer aliquots