Wisconsin: Lignin Project/27 June 2008

Checked the PCR product from last night on a gel and found several bands to work again (700bp).
The product and pBAD30 were digested with Xba1 and HindIII restriction enzymes.
They were verified to work on a gel and extracted using a Qiagen Gel Extraction Kit.
Unfortunately not enough DNA was obtained to perform a ligation.
Overnight culture started using revised C-Media recipe: growth after overnight incubation at 30C was observed
Single colony overnight culture started for growth curve following morning.
Team Fungus:
Plate with 1:3 insert to vector ratio had 2 colonies
Plate with 0:1 vector had 1 colony
Rest had zero colonies
We streaked and plated the two colonies from the 1:3 onto two new LB + Kan plates
Made more LB + Kan plates