User:University of Washington/8 August 2008

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pLux Regulation (Scott)

PCR of TrbA and KorA for making BioBrick parts
1.TrbA - pUB307
2.TrbA - RP4
3.KorA - pUB307
4.KorA - RP4

LuxR from AraC and TetR(Faifan)

-QuikChange trial#3 didn't seem to work. There was no growth in the reaction 1 and 2. There was no growth in negative(no primers) and growth in positive(no Dpn1) which said Dpn1 worked.

  • Ingrid set up new reactions(trial#4).
  • Tried tranformation again with 5 ul and 15 ul of DNA. (Last time used 2 ul), plated onto Amp.

-Miniprepped and sent sequencing for potential new BioBrick part: promoter AraC and TetR (from Elowitz) on pSB1AC3.

MG1655Z1

-Got lawn of E.coli on Tsy plates. The culture wasn't diluted enough(used 1:100 twice, supposed to be 1:1000 twice).

-Streaked out cultures from the plate with lawn of cells into new Tsy plates.


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