EPF-Lausanne/11 August 2008

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Molecular Biology

We try by different way to assemble biobrick

E1010 and F1610

We try last week to assemble two big parts because it is easy to purify it. We do ligation and transformation last week. The resultat was great all of the ten plates work. Monday, we decide to do a mini prep of these cells to purifiy DNA. Then we want to check the plasmide so we digest a small quantity of them with EcoRI and SpeI to prepare it to do a gel tomorrow.

by Gel

We try again to purifiy insert and vector by Gel. We try to assemble : R0040/B0034 R0071/B0034 and I1466/I1433 We successful have isolate from Gel : R0040, R0071 and B0034

http://openwetware.org/wiki/Knight:Annealing_and_primer_extension_with_Klenow_polymerase

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