"
EPF-Lausanne/12 August 2008
From 2008.igem.org
<<Previous - Back to Notebook - Next>>
Molecular Biology, assemblage of biobricks
All cells culture worked but unfortunately, there seems to have been a problem in the mini-prep procedure, as none of them have more than 30 ng/ul of DNA. So we redo new cell cultures once more.
F1610 and E1010
We do a gel to check if the ligation has worked (we cut the plasmids with SpeI and EcoRI to see the size of the insert). Unfortunately we don't see any fragment around the expected 1500 bp. However the positive control doesn't work either. The positive controls are F1610 (800 bp) and E1010 (700 bp). We decide to test the digestion (especially the enzymes) tommorrow, using both the E1010 and F1610 that we used for positive control and a new digestion of these Biobricks that we will do today. This way we will see if there is a problem in the digestion process.
R0040/B0034 and R0071/B0034
We do the transformation.
