Colony PCR
2 uL primer #1 (to 10uM)
2 uL primer #2
0.4 uL DNTPs
2 uL Buffer
5 uL colony culture
0.2 uL Taq
10.4 uL H20
92C 1:30 (92 0:30 50 :30 72 1:30) repeat x30 72 10:00
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