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Team:University of Lethbridge/Notebook/GeneralLabAugust
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August 13, 2008
Nathan Puhl and Sebastian
Verified and quantified the LacI and DT restriction digest products.
Set up a Ligase experiment for LacI and DT
- 1.3 uL vector(DT) - 8 uL insert(LacI) - 2 uL 10x Ligase buffer - 1.5 uL Ligase - 7.2 uL ddH2O - 20 uL total volume
August 14, 15
Nathan Puhl, Munima, Selina
Poured 61 LB + Amp plates. Stored in iGEM 4 C fridge.
August 16, 2008
Roxanne
Used Qiagen Plasmid MiniPrep Kit to Plasmid Prep Last Year's Biobrick Parts that were Incubated overnight.
Ran Plasmids in a 1% Agarose Gel at 100 V for 30 minutes.
August 19, 2008
Christa, Munima, Sebastian
Did a plasmid prep on pSB1A7 using QIAprep Spin Miniprep Kit. Stocked 4- 50uL of pSB1A7 and stored in iGEM -20 C.
