Team:The University of Alberta/18 August 2008

From 2008.igem.org

Revision as of 21:54, 18 August 2008 by Cwk (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Today

  • Site Directed Mutagenesis: Got LOTS of growth on the transformation control, a fair amount of growth on the mutagenesis control that was positive so we know that it works! HOWEVER, there was NO growth on the binar vector plates! What's going on?
    • It might be that the vector is simply too large to transform by heatshock into the cells (its nearlt 7kb!). We're going to try a regular transformation to see how if it works. If not, James suggested we try electroporation.
  • Retransformed pBS1A3 since it didnt work last time
Retrieved from "http://2008.igem.org/Team:The_University_of_Alberta/18_August_2008"